Showing papers by "Bryan R. Cullen published in 1980"

TL;DR: Using the technique of two-dimensional gel electrophoresis, a detailed study of the protein synthetic patterns of [35S]methionine-labeled preimplantation mouse embryos was undertaken, finding that, during the first day after fertilization, the pattern of newly synthesized protein changed dramatically.

TL;DR: Using high resolution two-dimensional polyacrylamide gel electrophoresis, the major protein species (labeled in vitro with 35S-methionine) of mouse embryos were examined starting with the unfertilized egg up to the blastocyst stage.

TL;DR: A computer program is described which facilitates comparison between the pattern of spots seen on different two-dimensional polyacrylamide electrophoresis gels by replotted on a graph by the computer using its molecular weight and isoelectric point as coordinates.