Showing papers by "Bulmaro Cisneros published in 1996"
TL;DR: The expression of dystrophin-protein 71 was investigated during nerve growth factor induced differentiation of PC12 cells as a suitable model for studying the function of Dp71 in neuronal cells.
22 citations
TL;DR: The results show that the intact hairpin of tl is essential for efficient transcription termination and for maintaining mRNA stability by blocking the 3′ to 5′ exonucleolytic activity of polynucleotide phosphorylase.
16 citations
Journal Article•
TL;DR: In order to improve carrier detection of Duchenne and Becker muscular dystrophy, dinucleotide sequences repeats (CA) of introns 44, 45, 49 and 50 were used as well as two markers located at the 5' and 3' ends of the dystrophin gene.
5 citations
01 Jan 1996
TL;DR: The upregulation of Dp71 expression during PC 12 cells differentiation point at PC12 cells as a suitable model for studying the function of D p71 in neuronal cells was investigated.
Abstract: The expression of dystrophin-protein 71 (Dp71) was investigated during nerve growth factor (NGF) induced differentiation of PC12 cells. A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was designed to measure Dp71 mRNA, whereas the Dp71 protein amount was evaluated by immunoblot analysis using an antidystrophin monoclonal antibody. comparison with control cultures showed that Dp71 mRNA and protein levels increased in parallel with NGF treatment peaking with increments of 60% and 1.4 times, respectively. The upregulation of Dp71 expression during PC12 cells differentiation point at PC12 cells as a suitable model for studying the function of Dp71 in neuronal cells. Keywords: Dystrophin-protein 71 expression; PC12 cells; Semiquantitative reverse transcription-polymerase chain reaction; Neuronal differentiation; Nerve growth factor Dystrophin, a large (427 kDa) protein which is absent or at very low levels in skeletal muscle of individuals suffer- ing from Duchenne muscular dystrophy (DMD) [1,13], is expressed mainly in muscle and the nervous system [1,21]. Predictions from the amino acid sequence suggest a rod- shaped triple-helical region separating an N-terminal act- ing binding region from a cysteine-rich and a C-terminal region [13,16]. Cellular localization of the dystrophin and its sequence similarities with other structural proteins such as a-actinin and spectrin indicate that it is a membrane- associated cytoskeletal protein
3 citations
01 Jan 1996
TL;DR: In this paper, dinucleotide sequences repeats (CA) of introns 44,45,49 and 50 were used as well as two markers located at the 5' and 3' ends of the dystrophin gene.
Abstract: In order to improve carrier detection of Duchenne and Becker muscular dystrophy, dinucleotide sequences repeats (CA) of introns 44,45,49 and 50 were used as well as two markers located at the 5' and 3' ends of the dystrophin gene. Haplotypes of the unaffected and affected persons of ten DXIDI KEY WORD: DMD; Carrier detection; PCR.