Carol A. Stremick

TL;DR: Minimal biofilm eradication concentrations, derived by using the Calgary Biofilm Device, demonstrated that for biofilms of the same organisms, 100 to 1,000 times the concentration of a certain antibiotic were often required for the antibiotic to be effective, while other antibiotics were found to beeffective at the MICs.

TL;DR: A core protocol and a set of parameters that can be adjusted to grow single- or multispecies bioFilms on peg surfaces that allows antimicrobial agents and exposure conditions to be tested against biofilms on a high-throughput scale are described.

TL;DR: The data indicate that growth in a biofilm does not provide resistance to bacteria against killing by metal cations or oxyanions, and metal compounds killed planktonic and biofilm cultures at the same concentration in the vast majority of combinations.

TL;DR: Results suggest that yafQ mediates the tolerance of E. coli biofilms to multiple but specific antibiotics; moreover, this data imply that this cellular pathway for persistence is likely different from that of multidrug-tolerant cells in stationary-phase planktonic cell cultures.

TL;DR: Several protocols for scanning electron microscopy and confocal laser scanning microscopy of biofilms grown on polystyrene pegs in the Calgary Biofilm Device (CBD), and a procedure is described for image processing of CLSM data stacks using amira™, a virtual reality tool, to create surface and/or volume rendered 3D visualizations of biofilm microorganisms.