C
Carola Engler
Publications - 21
Citations - 5959
Carola Engler is an academic researcher. The author has contributed to research in topics: Golden Gate Cloning & Molecular cloning. The author has an hindex of 17, co-authored 21 publications receiving 4879 citations.
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A one pot, one step, precision cloning method with high throughput capability.
TL;DR: A cloning strategy called ‘Golden Gate’ cloning was devised that allows to obtain in one tube and one step close to one hundred percent correct recombinant plasmids after just a 5 minute restriction-ligation, thus providing precision for this fundamental process of genetic manipulation.
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Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.
TL;DR: It is shown that one round of shuffling using the 27trypsinogen entry plasmids can easily produce the 19,683 different possible combinations in one single restriction-ligation and that expression screening of a subset of the library allows identification of variants that can lead to higher expression levels of trypsin activity.
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A Modular Cloning System for Standardized Assembly of Multigene Constructs
TL;DR: A hierarchical modular cloning system that allows the creation at will and with high efficiency of any eukaryotic multigene construct, starting from libraries of defined and validated basic modules containing regulatory and coding sequences.
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A Golden Gate Modular Cloning Toolbox for Plants
Carola Engler,Mark Youles,Ramona Gruetzner,Tim-Martin Ehnert,Stefan Werner,Jonathan D. G. Jones,Nicola J. Patron,Sylvestre Marillonnet +7 more
TL;DR: A versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation is presented.
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Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors.
Anatoli Giritch,Sylvestre Marillonnet,Carola Engler,Gerben van Eldik,Johan Botterman,Victor Klimyuk,Yuri Gleba +6 more
TL;DR: A rapid and indefinitely scalable process for high-level expression of functional full-size mAbs of the IgG class in plants, which allows production of gram quantities of mAbs for research purposes in just several days, and the same protocol can be used on an industrial scale in situations requiring rapid response, such as pandemic or terrorism events.