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Sylvestre Marillonnet
Researcher at Leibniz Association
Publications - 113
Citations - 11282
Sylvestre Marillonnet is an academic researcher from Leibniz Association. The author has contributed to research in topics: Gene & Golden Gate Cloning. The author has an hindex of 35, co-authored 110 publications receiving 9575 citations. Previous affiliations of Sylvestre Marillonnet include Norwich Research Park & John Innes Centre.
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A one pot, one step, precision cloning method with high throughput capability.
TL;DR: A cloning strategy called ‘Golden Gate’ cloning was devised that allows to obtain in one tube and one step close to one hundred percent correct recombinant plasmids after just a 5 minute restriction-ligation, thus providing precision for this fundamental process of genetic manipulation.
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Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.
TL;DR: It is shown that one round of shuffling using the 27trypsinogen entry plasmids can easily produce the 19,683 different possible combinations in one single restriction-ligation and that expression screening of a subset of the library allows identification of variants that can lead to higher expression levels of trypsin activity.
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A Modular Cloning System for Standardized Assembly of Multigene Constructs
TL;DR: A hierarchical modular cloning system that allows the creation at will and with high efficiency of any eukaryotic multigene construct, starting from libraries of defined and validated basic modules containing regulatory and coding sequences.
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The auxin influx carrier LAX3 promotes lateral root emergence
Kamal Swarup,Eva Benková,Eva Benková,Ranjan Swarup,Ilda Casimiro,Benjamin Péret,Yaodong Yang,Geraint Parry,Geraint Parry,Erik Nielsen,Ive De Smet,Ive De Smet,Steffen Vanneste,Mitch P. Levesque,Mitch P. Levesque,David John Carrier,Nicholas D. James,Vanessa Calvo,Karin Ljung,Eric M. Kramer,Rebecca Roberts,Neil S. Graham,Sylvestre Marillonnet,Kanu Patel,Jonathan D. G. Jones,Christopher G. Taylor,Daniel P. Schachtman,Sean T. May,Göran Sandberg,Philip N. Benfey,Jiri Friml,Jiri Friml,Ian D. Kerr,Tom Beeckman,Laurent Laplaze,Malcolm J. Bennett +35 more
TL;DR: It is described how the hormone auxin, which originates from the developing lateral root, acts as a local inductive signal which re-programmes adjacent cells in cortical and epidermal cells directly overlaying new primordia.
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A Golden Gate Modular Cloning Toolbox for Plants
Carola Engler,Mark Youles,Ramona Gruetzner,Tim-Martin Ehnert,Stefan Werner,Jonathan D. G. Jones,Nicola J. Patron,Sylvestre Marillonnet +7 more
TL;DR: A versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation is presented.