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Nicola J. Patron
Researcher at Norwich Research Park
Publications - 86
Citations - 5474
Nicola J. Patron is an academic researcher from Norwich Research Park. The author has contributed to research in topics: Synthetic biology & Gene. The author has an hindex of 30, co-authored 80 publications receiving 4517 citations. Previous affiliations of Nicola J. Patron include University of Cambridge & University of British Columbia.
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Journal ArticleDOI
Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote
Jonathan A. Eisen,Robert S. Coyne,Martin Wu,Dongying Wu,Mathangi Thiagarajan,Jennifer R. Wortman,Jonathan H. Badger,Qinghu Ren,Paolo Amedeo,Kristie M. Jones,Luke J. Tallon,Arthur L. Delcher,Steven L. Salzberg,Joana C. Silva,Brian J. Haas,William H. Majoros,Maryam Farzad,Jane M. Carlton,Jane M. Carlton,Roger Smith,Jyoti Garg,Ronald E. Pearlman,Kathleen M Karrer,Lei Sun,Gerard Manning,Nels C. Elde,Aaron P. Turkewitz,David J. Asai,David E. Wilkes,Yufeng Wang,Hong-Hong Cai,Kathleen E Collins,B. Andrew Stewart,Suzanne R. Lee,Katarzyna Wilamowska,Zasha Weinberg,Walter L. Ruzzo,Dorota Wloga,Jacek Gaertig,Joseph Frankel,Che-Chia Tsao,Martin A. Gorovsky,Patrick J. Keeling,Ross F. Waller,Nicola J. Patron,J. Michael Cherry,Nicholas A. Stover,Cynthia J. Krieger,Christina del Toro,Hilary F. Ryder,Sondra Williamson,Rebecca A Barbeau,Eileen P. Hamilton,Eduardo Orias +53 more
TL;DR: The T. thermophila genome has been used for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance as mentioned in this paper, and it has been shown that the excision of DNA from the MIC to generate the macronucleus specifically targets foreign DNA as a form of genome self-defense.
Journal ArticleDOI
A Golden Gate Modular Cloning Toolbox for Plants
Carola Engler,Mark Youles,Ramona Gruetzner,Tim-Martin Ehnert,Stefan Werner,Jonathan D. G. Jones,Nicola J. Patron,Sylvestre Marillonnet +7 more
TL;DR: A versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation is presented.
Journal ArticleDOI
Editing plant genomes with CRISPR/Cas9.
TL;DR: Together with other sequence-specific nucleases, CRISPR/Cas9 is a game-changing technology that is poised to revolutionise basic research and plant breeding.
Journal ArticleDOI
Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease
Tom Lawrenson,Oluwaseyi Shorinola,Nicola Stacey,Chengdao Li,Lars Østergaard,Nicola J. Patron,Cristobal Uauy,Wendy Harwood +7 more
TL;DR: The use of RNA-guided Cas9 is demonstrated to generate mutations in target genes of both barley and B. oleracea and show stable transmission of these mutations thus establishing the potential for rapid characterisation of gene function in these species.
Journal ArticleDOI
Standards for plant synthetic biology: a common syntax for exchange of DNA parts.
Nicola J. Patron,Nicola J. Patron,Diego Orzaez,Sylvestre Marillonnet,Heribert Warzecha,Colette Matthewman,Colette Matthewman,Mark Youles,Oleg Raitskin,Oleg Raitskin,Aymeric Leveau,Gemma Farré,Christian Rogers,Alison G. Smith,Julian M. Hibberd,Alex A. R. Webb,James C. W. Locke,Sebastian Schornack,Jim Ajioka,David C. Baulcombe,Cyril Zipfel,Sophien Kamoun,Jonathan D. G. Jones,Hannah Kuhn,Silke Robatzek,H. Peter van Esse,Dale Sanders,Dale Sanders,Giles E. D. Oldroyd,Giles E. D. Oldroyd,Cathie Martin,Cathie Martin,Robert A. Field,Robert A. Field,Sarah E. O'Connor,Sarah E. O'Connor,Samantha Fox,Brande B. H. Wulff,Ben Miller,Andy Breakspear,Guru V. Radhakrishnan,Pierre-Marc Delaux,Dominique Loqué,Dominique Loqué,Antonio Granell,Alain Tissier,Patrick M. Shih,Thomas P. Brutnell,W. Paul Quick,Heiko Rischer,Paul D. Fraser,Asaph Aharoni,Christine A. Raines,Paul F. South,Jean-Michel Ané,Björn Hamberger,Jane A. Langdale,Jens Stougaard,Harro J. Bouwmeester,Michael K. Udvardi,James A. H. Murray,Vardis Ntoukakis,Patrick Schäfer,Katherine J. Denby,Keith J. Edwards,Anne Osbourn,Anne Osbourn,Jim Haseloff +67 more
TL;DR: A standard for Type IIS restriction endonuclease-mediated assembly is described, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units.