Carolyn A. Joswig-Jones

TL;DR: Human aldehyde oxidase 1 has been subcloned into a vector suitable for expression in Escherichia coli, and the protein has been expressed and is active, with sulfur being incorporated in the molybdopterin cofactor.

TL;DR: This work developed and employed a trypsin digest and subsequent liquid chromatography-tandem mass spectrometry analysis to determine absolute amounts of AOX1 in human liver, and measured a large difference in kcat/Km, indicating cytosol to be 11-14 times more efficient in the turnover of DACA than the E. coli expressed purified enzyme.

TL;DR: Molecular docking of methotrexate into the active site of the enzyme led to the identification of important residues that could potentially be involved in substrate binding and account for the observed differences in activity.

TL;DR: Investigation of the in vitro metabolism of 6MP to 6-thiouric acid (6TUA) in pooled human liver cytosol found evidence that three enzymes, AO, XO, and XDH, contribute to the production of 6TX intermediate, whereas only XO andXDH are involved in the conversion of 6 TX to 6TUA in pooled HLC.

TL;DR: It is shown that type II binding can have a profound influence on binding affinity for CYP3A4, and the difference in binding affinity can be as high as 1200-fold.