C
Caterina Temporini
Researcher at University of Pavia
Publications - 76
Citations - 1728
Caterina Temporini is an academic researcher from University of Pavia. The author has contributed to research in topics: Immobilized enzyme & Hydrophilic interaction chromatography. The author has an hindex of 24, co-authored 70 publications receiving 1520 citations.
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Journal ArticleDOI
Development of a bioreactor based on trypsin immobilized on monolithic support for the on-line digestion and identification of proteins
Enrica Calleri,Caterina Temporini,Eleonora Perani,Cinzia Stella,Serge Rudaz,Dieter Lubda,Giorgio Mellerio,Jean-Luc Veuthey,Gabriele Caccialanza,Gabriella Massolini +9 more
TL;DR: The preparation and characterization of a new trypsin-based bioreactor is here described for on-line protein digestion and peptide analysis and the influence of various parameters (flow rate, temperature, buffer pH and molarity, etc.) on enzymatic activity was investigated and the mostly significant factor was found to be the flow rate.
Journal ArticleDOI
Integrated analytical strategies for the study of phosphorylation and glycosylation in proteins.
TL;DR: This review focuses on the description of recent integrated analytical systems proposed for the analysis of PTMs in proteins, and their application to profile the glycoproteome and the phosphoproteome in biological samples.
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Trypsin-based monolithic bioreactor coupled on-line with LC/MS/MS system for protein digestion and variant identification in standard solutions and serum samples.
Enrica Calleri,Caterina Temporini,Eleonora Perani,Antonella De Palma,Dieter Lubda,Giorgio Mellerio,Alberto Sala,Monica Galliano,Gabriele Caccialanza,Gabriella Massolini +9 more
TL;DR: By digesting picomoles of proteins sufficient data from ESI and MS/MS were obtained to unambiguously identify proteins alone and in serum samples, and this approach was also extended to locate mutation sites in beta-lactoglobulin A and B variants.
Journal ArticleDOI
Evaluation of a monolithic epoxy silica support for penicillin G acylase immobilization.
Enrica Calleri,Gabriella Massolini,Dieter Lubda,Caterina Temporini,Fulvio Loiodice,Gabriele Caccialanza +5 more
TL;DR: A new type of penicillin G acylase (PGA)-based monolithic silica support was developed and evaluated for the chiral separation in HPLC and the enantioselectivity and the performance of the developed column are discussed and compared to the corresponding experimental data obtained.
Journal ArticleDOI
Frontal affinity chromatography-mass spectrometry useful for characterization of new ligands for GPR17 receptor.
Enrica Calleri,Stefania Ceruti,Gloria Cristalli,Claudia Martini,Caterina Temporini,Chiara Parravicini,Rosaria Volpini,Simona Daniele,Gabriele Caccialanza,Davide Lecca,Catia Lambertucci,Maria Letizia Trincavelli,Gabriella Marucci,Irving W. Wainer,Graziella Ranghino,Piercarlo Fantucci,Maria P. Abbracchio,Gabriella Massolini +17 more
TL;DR: In this paper, frontal affinity chromatography-mass spectrometry (FAC-MS) was applied to the screening of potential drug candidates toward G-protein-coupled receptor (GPCR) GPR17.