Serge Rudaz

TL;DR: Advantages and problems encountered with high pressure in terms of frictional heating and solvent compressibility will be discussed even if systems working at a maximum pressure of 1000 bar are not influenced by these parameters and give reliable and reproducible results.

TL;DR: Among the different off-line sample preparations, LLE was the most efficient extraction procedure and on-line SPE-LC-MS configuration exhibited matrix effects, which depend on the API source and the extraction support.

TL;DR: A new metabolite profiling approach combined with an ultrarapid sample preparation procedure was used to study the temporal and spatial dynamics of the wound-induced accumulation of jasmonic acid and its oxygenated derivatives in Arabidopsis thaliana, finding changes occurred throughout the plant and were not restricted to wounded leaves.

TL;DR: This study demonstrates that the best analytical strategy should be selected on the basis of the analytical problem (e.g., isocratic vs. gradient, throughput vs. efficiency) and the properties of the analyte.

TL;DR: Applications of special extraction sorbents, such as restricted access media (RAM) and large particle supports (LPS), allowing the direct and repetitive injection of complex biological matrices, lead to the automation, simplification and speeding up of the sample preparation process.