There is, I think, something ethereal about i —the square root of minus one. I remember first hearing about it at school. It seemed an odd beast at that time—an intruder hovering on the edge of reality.

Usually familiarity dulls this sense of the bizarre, but in the case of i it was the reverse: over the years the sense of its surreal nature intensified. It seemed that it was impossible to write mathematics that described the real world in …

I and i

THE JOURNAL OF BIOLOGICAL CHEMISTRY: The Biochemical Behavior of LeadL I. Influence of Calcium, Phosphorus, and Vitamin D on Lead in Blood and Bone

Recent developments in biological activities of chalcones: a mini review.

/pdf/a-review-on-versatile-applications-of-transition-metal-4bf9r8gsd3.pdf

A review on versatile applications of transition metal complexes incorporating Schiff bases

Medicinal prospects of antioxidants: A review

Synthesis and biological evaluation of simple methoxylated chalcones as anticancer, anti-inflammatory and antioxidant agents

L-asparaginase was extracted from Erwinia carotovora and purified by ammonium sulfate fractionation (60–70%), Sephadex G-100, CM cellulose, and DEAE sephadex chromatography. The apparent Mr of enzyme under nondenaturing and denaturing conditions was 150 kDa and 37±0.5 kDa, respectively. L-asparaginase activity was studied in presence of thiols, namely, L-cystine (Cys), L-methionine (Met), N-acetyl cysteine (NAC), and reduced glutathione (GSH). Kinetic parameters in presence of thiols (10–400 𝜇M) showed an increase in Vmax values (2000, 2223, 2380, 2500, and control 1666.7 𝜇moles mg−1min−1) and a decrease in K𝑚 values (0.086, 0.076, 0.062, 0.055 and control 0.098 mM) indicating nonessential mode of activation. KA values displayed propensity to bind thiols. A decrease in Vmax/K𝑚 ratio in concentration plots showed inverse relationship between free thiol groups (NAC and GSH) and bound thiol group (Cys and Met). Enzyme activity was enhanced in presence of thiol protecting reagents like dithiothreitol (DTT), 2-mercaptoethanol (2-ME), and GSH, but inhibited by p-chloromercurybenzoate (PCMB) and iodoacetamide (IA).

/pdf/purification-characterization-and-effect-of-thiol-compounds-357iohnex5.pdf

Purification, Characterization, and Effect of Thiol Compounds on Activity of the Erwinia carotovora L-Asparaginase

http://www.scielo.br/pdf/bjm/v40n2/v40n2a28.pdf

Purification and characterization of extracellular lipase from a new strain: Pseudomonas aeruginosa SRT 9

Synthesis and antimicrobial activity of novel pyrazolo[3,4-d]pyrimidin derivatives.

L-Asparaginase shows antileukemic activity and is generally administered in the body in combination with other anticancer drugs like pyrimidine derivatives. In the present study, L-asparaginase was purified from a bacteria Erwinia carotovora and the effect of a dihydropyrimidine derivative (1-amino-6-methyl-4-phenyl-2-thioxo, 1,2,3,4-tetrahydropyrimidine-5-carboxylic acid methyl ester) was studied on the kinetic parameters Km and Vmax of the enzyme using L-asparagine as substrate. The enzyme had optimum activity at pH 8.6 and temperature 35 degrees C, both in the absence and presence of pyrimidine derivative and substrate saturation concentration at 6 mg/ml. For the enzymatic reaction in the absence and presence (1 to 3 mg/ml) of dihydropyrimidine derivative, Km values were 7.14, 5.26, 4.0, and 5.22 M, and Vmax values were 0.05, 0.035, 0.027 and 0.021 mg/ml/min, respectively. The kinetic values suggested that activity of enzyme was enhanced in the presence of dihydropyrimidine derivative.

http://nopr.niscair.res.in/bitstream/123456789/3412/1/IJBB%2043%286%29%20391-394.pdf

Chandrahas N. Khobragade

Papers

Synthesis and biological evaluation of simple methoxylated chalcones as anticancer, anti-inflammatory and antioxidant agents

Purification, Characterization, and Effect of Thiol Compounds on Activity of the Erwinia carotovora L-Asparaginase

Purification and characterization of extracellular lipase from a new strain: Pseudomonas aeruginosa SRT 9

Synthesis and antimicrobial activity of novel pyrazolo[3,4-d]pyrimidin derivatives.

Purification of L-asparaginase from a bacteria Erwinia carotovora and effect of a dihydropyrimidine derivative on some of its kinetic parameters.