Capping of eucaryotic mRNAs

This review concern recent experimental information areas of animal cell molecular biology which are relevant to the mechanism of gene regulation. New data regarding interspersion and clustering of repetitive sequence elements in DNA are considered Molecular characteristics of
animal structural genes and mRNAs are discussed, with particular reference the frequency of structural gene sequences, mRNA turn over and the interpretation of dipteran complementation groups. The molecular characteristics of nuclear RNAs, the primary transcription products, are reviewed. Evidence for transcription level regulation is summarized and the relation of nuclear
and mRNA examined. The protein activator branch of the Britten-Davidson model for gene regulation is further developed and considered in light of current knowledge.

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Organization, transcription, and regulation in the animal genome.

Messenger RNAs from mouse myeloma cells contain N6-methyl adenosine and novel 5′ termini having 7-methylguanosine in a 5′, 5′triphosphate linkage with ribose-methylated nucleosides. Ten common 5′-terminal sequences of the forms m7G5′ppp5′NmpNp and m7G5′ppp5′NmpNmpNp are reported. Structures like this may be a general feature of mRN A in eukaryotes.

Modified nucleosides and bizarre 5'-termini in mouse myeloma mRNA.

The model suggests that chromosomal DNA falls into two classes: globular DNA (containing unpaired regions for control) and a much smaller fraction consisting of fibrous DNA which alone codes for proteins.

General Model for the Chromosomes of Higher Organisms

Functional expression of plant plasma membrane H(+)-ATPase in yeast endoplasmic reticulum.

On the structure of transcriptional unit in mammalian cells.

https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2870%2980584-1

Localization of messenger RNA near the 3'-end of the dRNA precursor molecule.

Melted ds RNA isolated from rabbit bone marrow pre-mRNA was hybridized with excess of globin mRNA which was prepared from rabbit reticulocytes 7-9% of ds sequences became RNAase-stable and about 30% of the sequences could be bound to poly(U)-Sepharose through poly (A) of mRNA The size of RNAase-stable hybrid is about 30 nucleotides, that is one fourth of the length of one strand of the ds RNA

Charles Coutelle

Papers

On the structure of transcriptional unit in mammalian cells.

Localization of messenger RNA near the 3'-end of the dRNA precursor molecule.

Globin mRNA contains a sequence complementary to double-stranded region of nuclear pre-mRNA.