Capping of eucaryotic mRNAs

This review concern recent experimental information areas of animal cell molecular biology which are relevant to the mechanism of gene regulation. New data regarding interspersion and clustering of repetitive sequence elements in DNA are considered Molecular characteristics of
animal structural genes and mRNAs are discussed, with particular reference the frequency of structural gene sequences, mRNA turn over and the interpretation of dipteran complementation groups. The molecular characteristics of nuclear RNAs, the primary transcription products, are reviewed. Evidence for transcription level regulation is summarized and the relation of nuclear
and mRNA examined. The protein activator branch of the Britten-Davidson model for gene regulation is further developed and considered in light of current knowledge.

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Organization, transcription, and regulation in the animal genome.

Messenger RNAs from mouse myeloma cells contain N6-methyl adenosine and novel 5′ termini having 7-methylguanosine in a 5′, 5′triphosphate linkage with ribose-methylated nucleosides. Ten common 5′-terminal sequences of the forms m7G5′ppp5′NmpNp and m7G5′ppp5′NmpNmpNp are reported. Structures like this may be a general feature of mRN A in eukaryotes.

E. R. Avakyan

Papers

On the structure of transcriptional unit in mammalian cells.