Showing papers by "Charles Huggins published in 1934"
TL;DR: The formation of dentin and enamel in the abdominal wall in young pups was achieved by transplantation of the soft tissues of the developing tooth germ and an interesting finding was the cytomorphosis of the epithelium of the enamel organ.
Abstract: The formation of dentin and enamel in the abdominal wall in young pups was achieved by transplantation of the soft tissues of the developing tooth germ. An interesting finding was the cytomorphosis of the epithelium of the enamel organ. When this was transplanted so that the ameloblasts were in contact with the odontoblasts the cylindrical character of the epithelial cells was preserved and enamel was produced; otherwise the cylindrical shape of these cells was lost and a stratified epithelium resulted, resembling the gingival and certain tumors (the adamantinoma) of the jaw and related structures. This degenerated epithelium did not produce enamel and had an important characteristic of not forming cysts in a closed connective tissue space, instead forming islands and cords of cells with epithelial pearl formation. Thus the influence of mesodermic connective tissue derivatives on the form and function of epithelium is presented. The odonto-blasts were found capable of survival as such and readily formed new dentin in transplantation; the stellate cells of the pulp were inert from the standpoint of inducing calcification.
81 citations
6 citations
TL;DR: Dental epithelium surrounding the base of the permanent cuspid teeth, and pulp tissue in 18 young dogs were transplanted to the fascia of the abdominal wall as an autogenous graft, and after 9 to 31 days there was found newly formed enamel, dentine, cementum and bone.
Abstract: Epithelium surrounding the base of the permanent cuspid teeth, and pulp tissue in 18 young dogs were transplanted to the fascia of the abdominal wall as an autogenous graft, and after 9 to 31 days there was found newly formed enamel, dentine, cementum and bone.Method. The epithelium was obtained in the following way: Under ether anesthesia a cuneiform osteotomy of the mandible, including the deciduous cuspid tooth was made in dogs of between 3 and 6 weeks of age and this allowed access to the permanent cuspid tooth, which was removed. A circular incision was made equatorially allowing the dental epithelium and pulp to be removed en masse from the calcified tooth shell. The tooth shell itself was always discarded. The soft tissues were gently wiped with moist gauze, were X-rayed, and a section was removed for histological control. The calcium-free material was then transplanted to a pocket between the lateral abdominal muscles, with aseptic pre cautions, and the incision closed with silk.It was found easy ...
3 citations