C
Chen Hsu
Publications - 5
Citations - 1482
Chen Hsu is an academic researcher. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 2, co-authored 2 publications receiving 1427 citations.
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Journal ArticleDOI
Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources
Chih-Ching Chu,Ching-Chu Wang,Ching-San Sun,Chen Hsu,Kwang-Chu Yin,Chih--Yin Chu,Feng-Yun Bi +6 more
TL;DR: In this paper, it was shown that (NH4)2SO4 combined with KNO3 at low concentration is of advantage to the formation, growth and differentiation of pollen callus in rice.
The androgenesis in wheat (triticum aestivum) anthers cultured in vitro
TL;DR: The differentiation of young shoots from callus was found to depend not only on the medium employed but also on the feature of the callus cells, which produced morphologically irregular callus.
Journal ArticleDOI
DNA-free CRISPR-Cas9 gene editing of wild tetraploid tomato Solanum peruvianum using protoplast regeneration
Choun-Sea Lin,Chen Hsu,Yuehua Yuan,Po-Xing Zheng,Fubing Wu,Qiao-Wei Cheng,Yu-Lin Wu,Ting-Li Wu,Steven Lin,Jin-Jun Yue,Ying-Huey Cheng,Ming-Che Shih,Jen Sheen,Yao-Cheng Lin +13 more
TL;DR: A DNA-free CRISPR-Cas9 genome editing system based on an optimized protoplast regeneration protocol of S. peruvianum is established and all mutated genes in both diploid and tetraploid regenerants were heritable in the next generation.
Book ChapterDOI
Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum.
TL;DR: The Nicotiana tabacum transient protoplast transfection and regeneration system can effectively obtain target gene mutations in regenerated plants without transgenes and is thus a very attractive technique for evaluating gene editing reagents using CRISPR/Cas-based systems as discussed by the authors .
Book ChapterDOI
Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration.
Fubing Wu,Chen Hsu,Choun-Sea Lin +2 more
TL;DR: In this paper , a simple protocol that eliminates the need for expensive equipment, chemicals, modifications of donor DNA, and complicated vector construction was developed, which used polyethylene glycol (PEG)-calcium to deliver low-cost, unmodified single-stranded oligodeoxyribonucleotides and CRISPR/Cas9 ribonucleoprotein (RNP) complexes into Nicotiana benthamiana protoplasts.