C
Chester E. Holmlund
Researcher at University of Maryland, College Park
Publications - 19
Citations - 279
Chester E. Holmlund is an academic researcher from University of Maryland, College Park. The author has contributed to research in topics: Tetrahymena pyriformis & Ergosterol. The author has an hindex of 11, co-authored 19 publications receiving 277 citations. Previous affiliations of Chester E. Holmlund include National Institutes of Health.
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Isolation of 2,3;22,23-dioxidosqualene and 24,25-oxidolanosterol from yeast.
TL;DR: The compound 2,3;22,23-dioxidosqualene has been found to accumulate in yeast grown in the presence of 10−4m 3β-(β-dimethylaminoethoxy)-androst-5-en-17-one and subsequent removal of the latter compound permits the formation of 24,25-oxidolanosterol and more polar unidentified compounds.
Journal ArticleDOI
Extraction of lipids from yeast
M. T. Sobus,Chester E. Holmlund +1 more
TL;DR: A preparative method involving preincubation of cells with aqueous KOH followed by the treatment of the cellular residue yielded slightly more steryl esters than was extracted from broken cell preparations.
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Purification and kinetic characterization of a specific glucokinase from Streptococcus mutans OMZ70 cells
TL;DR: In this paper, the authors showed that glucose 6-phosphate was not an inhibitor toward ATP at saturating glucose concentrations, but exhibited noncompetitive inhibition at subsaturating glucose concentration.
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The effect of triparanol on the composition of free and esterified sterols of Saccharomyces cerevisiae.
TL;DR: The authors showed that triparanol altered the sterol composition of Saccharomyces cerevisiae and promoted an increase in the steryl ester and total sterol per organism.
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Effect of triparanol and 3β-(β-dimethyl-aminoethoxy)-androst-5-en-17-one on growth and non-saponifiable lipids of saccharomyces cerevisiae
Bess Fung,Chester E. Holmlund +1 more
TL;DR: Preliminary observation suggests that major effects caused by triparanol on the incorporation of [1− 14 C] sodium acetate into non-saponifiable lipids involved interference with the conversion of lanosterol to ergosterol, and to a lesser extent inhibition of squalene oxidation.