Christina Priemer

TL;DR: It is demonstrated that a highly stressful event in the absence of systemic inflammation, as observed in patients with acute myocardial infarction, leads to the development of an active HCMV infection in latently infected patients, and suggests that H CMV, like HSV-1 and VZV, can be (re)activated under stress conditions.

TL;DR: A protocol for chemiluminescence detection of hantaviral antigens in infected cell foci is described, which enables reduction of incubation time of virus-infected cells and amount of needed antibody for antigen detection in foci.

TL;DR: Proteasome inhibitors like MG132, PSI, II and III (MG262) have been shown to block both TNF-alpha-associated up-regulation of the H CMV IE1/2 enhancer/promoter in monocytic cells in an in vitro transient transfection system and HCMV replication in permissive embryonal fibroblasts, which suggests that ganciclovir-resistant HCMv strains are sensitive to proteasome inhibition.

TL;DR: The virus inactivation and depletion methods described herein are suitable to prepare non-infectious samples for further use in immunological, virological and cell-biological assays.

TL;DR: In transient transfection experiments using HCMV immediate early enhancer/promoter CAT reporter gene constructs PTX enhanced the promoter activity independently from TNF alpha in premonocytic cells, suggesting a potential side effect of cAMP-elevating drugs such as PTX.