Showing papers in "Virology in 2000"

TL;DR: A review of cell surface receptors for alpha-herpesvirus entry can be found in this paper, where the authors show that deletion of genes for any one of these four glycoproteins is lethal.

TL;DR: The available evidence supports the notion of differences in pathogenicity of H9N2 viruses in the different lineages and suggests that viruses possessing genome segments similar to 1997 H5N1-like viruses are potentially pathogenic in mammals.

TL;DR: A rigorous analysis of assembly of assembly to cowpea chlorotic mottle virus, a typical, small, positive-strand RNA virus, finds that the capsid protein readily forms pentamers of dimers in solution, based on polymerization kinetics observed by light scattering.

TL;DR: Phylogenetic analysis demonstrated that although HV and NV are closely related, they are clearly distinct from any of the established genera within the Paramyxoviridae and should be considered a new genus.

TL;DR: The hypothesis that HIV-1 infection alters expression of a broad array of cellular genes and provides a framework for future functional studies on the differentially expressed mRNA products is supported.

TL;DR: Results demonstrate for the first time that HS-GAGs expressed on the cell surface are involved in the binding of Ad5 and Ad2 to host cells.

TL;DR: Together, the data suggested that CTGV may have derived from VV-IOC by persisting in an indigenous animal(s), accumulating polymorphisms, and now emerging in cattle and milkers as CTGV, the first case of long-term persistence of vaccinia in the New World.

TL;DR: This data suggests that unlike VLP-based prophylactic HPV vaccines, an L2 polypeptide vaccine may provide broad-spectrum protection in animals against experimental challenge with homologous papillomavirus and cross-reactive epitopes.

TL;DR: It is hypothesized that SPCSV is able to enhance the multiplication of SPFMV in tissues other than where it occurs itself, perhaps by interfering with systemic phloem-dependent signaling required in a resistance mechanism directed against SP FMV.

TL;DR: Data indicate that KS and PEL cells expressed predominantly latent proteins, whereas MCD expressed both latent and lytic proteins, suggesting that HHV8 plays a different role in the pathogenesis of HHV 8-associated diseases.

TL;DR: It is established that the GAGs involved in RSV infection are present on the cell rather than on the virus particle, and the presence of cell surface G AGs containing iduronic acid, like heparan sulfate and chondroitin sulfate B, is required for efficient RSV infections in cell culture.

TL;DR: It is proposed that one mechanism of attenuation is rapid viral clearance in vivo due to binding of the virus to ubiquitous heparan sulfate.

TL;DR: Although interference with IFN signaling is a common strategy among Paramyxovirinae, distinct virus-specific mechanisms are used to achieve this end, demonstrating that RSV must have an alternative method for circumventing the IFN response.

TL;DR: These major viral proteins showed no homology to baculovirus structural proteins, suggesting that WSSV may be a representative of a new virus family, Whispoviridae.

TL;DR: Subgenomic RNA ynthesis has been studied more in plant viruses than in nimal viruses, probably because a greater perentage of all plant viruses make sgRNAs and also ecause plant viruses are often more amenable than anial viruses to studies of RNA replication mechanisms, specially in cell-free extracts.

TL;DR: It is shown for the first time that inflammatory cytokines can directly modulate KSHV replication and the identification of interferon-gamma as an activator and interfer on-alpha as an inhibitor of K SHV induction in vitro correlates well with in vivo observations and demonstrates for theFirst time thatinflammatory cytokinesCan directly modulates KSHv replication.

TL;DR: Results indicate that NDV can be engineered to express foreign protein stably and can be manipulated in the future for use as a vaccine vector and introduced an additional gene into the NDV genome to achieve growth retardation and attenuation.

TL;DR: Results indicate that the GP ectodomain and its anchorage to the membrane are required for GP-induced morphologic changes in host cells, and suggest that integrins, especially the beta1 group, might interact with the GP and perhaps be involved in Ebola virus entry into cells.

TL;DR: The results indicated that vaccination with both L1R and A33R proteins, intended to evoke mechanistically distinct and complementary forms of protection, was more effective than vaccination with either protein by itself.

TL;DR: Direct evidence is provided that E6 and E7 contribute differently to carcinogenesis; E7 promotes the formation of benign tumors, and E6 acts primarily to accelerate progression of these benign tumors to the malignant stage.

TL;DR: The utility of this approach is proved by the generation of virus after transfecting the pol I/pol II-promoter-PB1 construct together with vRNA- and protein-expression constructs for the remaining seven segments, and the use of the reverse-genetics system to cell lines for which efficient cotransfection of 17 plasmids cannot be achieved.

TL;DR: The involvement of gene products of Citrus tristeza virus (CTV) in virion formation was examined and it was found that the chaperone-like protein plus the p61 and both coat proteins were required for efficient virion assembly.

TL;DR: The data indicated that the NS1, NS2, SH, and M2-2 proteins, although dispensable for virus replication in vitro, provide auxiliary functions for efficient RSV replication.

TL;DR: Serological, sequence, and in vitro host range analyses of feline parvovirus isolates in Vietnam and Taiwan indicated the possibility that CPV-2a/2b-type viruses can spread in cats more efficiently than conventional FPLV under natural conditions and that CPVs are further evolving in cats.

TL;DR: Rec recombinant P56 was expressed as a hexahistidine-tagged protein in Escherichia coli and purified it to apparent homogeneity using affinity chromatography to show that P56 is primarily a cytoplasmic protein.

TL;DR: In vivo, in vivo, using YAC-CD46 transgenic mice as a model for MV induced CNS disease, MV C and V proteins encode virulence functions in vivo and likely operate via separate mechanisms.

TL;DR: New information has been recently obtained on the two sides of the survival line: the edge of adaptation and theedge of extinction.

TL;DR: The conservation of this previously unrecognized open reading frame with homology to interleukin-10 suggests that the protein may play an essential role in primate CMV persistence and pathogenesis.