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Christopher D. Guzman
Researcher at Yale University
Publications - 10
Citations - 2209
Christopher D. Guzman is an academic researcher from Yale University. The author has contributed to research in topics: CRISPR & Gene. The author has an hindex of 6, co-authored 10 publications receiving 1558 citations. Previous affiliations of Christopher D. Guzman include Massachusetts Institute of Technology & Wyss Institute for Biologically Inspired Engineering.
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Journal ArticleDOI
Highly efficient Cas9-mediated transcriptional programming
Alejandro Chavez,Jonathan Scheiman,Suhani Vora,Benjamin W. Pruitt,Marcelle Tuttle,Eswar Prasad Ramachandran Iyer,Shuailiang Lin,Samira Kiani,Christopher D. Guzman,Daniel J. Wiegand,Dmitry Ter-Ovanesyan,Jonathan L. Braff,Noah Davidsohn,Benjamin E. Housden,Norbert Perrimon,Ron Weiss,John Aach,James J. Collins,George M. Church +18 more
TL;DR: An improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9 is described and demonstrated in activating endogenous coding and noncoding genes and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).
Highly efficient Cas9-mediated transcriptional programming
Alejandro Chavez,Jonathan Scheiman,Marcelle Tuttle,Shuailiang Lin,Samira Kiani,Dmitry Ter-Ovanesyan,Noah Davidsohn,Norbert Perrimon,Ron Weiss,John Aach,Suhani Vora,Benjamin W. Pruitt,Eswar Prasad Ramachandran Iyer,Christopher D. Guzman,Daniel J. Wiegand,Jonathan L. Braff,Benjamin E. Housden,James J. Collins,George M. Church +18 more
TL;DR: In this article, the authors describe the development of an improved transcriptional regulator through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to Cas9 and demonstrate its utility in activating expression of endogenous coding and non-coding genes, targeting several genes simultaneously and stimulating neuronal differentiation of induced pluripotent stem cells (iPSCs).
Posted ContentDOI
Highly-efficient Cas9-mediated transcriptional programming
Alejandro Chavez,Jonathan Scheiman,Suhani Vora,Benjamin W. Pruitt,Marcelle Tuttle,Eswar Prasad Ramachandran Iyer,Samira Kiani,Christopher D. Guzman,Daniel J. Wiegand,Dmitry Ter-Ovanesyan,Jonathan L. Braff,Noah Davidsohn,Ron Weiss,John Aach,James J. Collins,George M. Church +15 more
TL;DR: The development of an improved transcriptional regulator through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to Cas9 is described and its utility in activating expression of endogenous coding and non-coding genes, targeting several genes simultaneously and stimulating neuronal differentiation of induced pluripotent stem cells (iPSCs).
Journal ArticleDOI
Synthetic biosensors for precise gene control and real-time monitoring of metabolites
Jameson K. Rogers,Christopher D. Guzman,Noah D. Taylor,Srivatsan Raman,Kelley Anderson,George M. Church +5 more
TL;DR: This work applies a characterization pipeline to four genetically encoded sensors that respond to acrylate, glucarate, erythromycin and naringenin, and shows that activation of each sensor is orthogonal to the other sensors, and to other common inducible systems.
Journal ArticleDOI
AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma
Ryan D. Chow,Christopher D. Guzman,Guangchuan Wang,Florian I. Schmidt,Florian I. Schmidt,Mark W. Youngblood,Lupeng Ye,Youssef Errami,Matthew B. Dong,Michael A. Q. Martinez,Sensen Zhang,Paul A. Renauer,Kaya Bilguvar,Murat Gunel,Phillip A. Sharp,Feng Zhang,Randall Jeffrey Platt,Randall Jeffrey Platt,Sidi Chen +18 more
TL;DR: This study provides a functional landscape of gliomagenesis suppressors in vivo and identifies co-occurring driver combinations such as B2m–Nf1, Mll3–N f1 and Zc3h13–Rb1, which were subsequently validated using AAV minipools.