D
Dmitry Ter-Ovanesyan
Researcher at Wyss Institute for Biologically Inspired Engineering
Publications - 23
Citations - 3847
Dmitry Ter-Ovanesyan is an academic researcher from Wyss Institute for Biologically Inspired Engineering. The author has contributed to research in topics: Extracellular vesicle & Gene. The author has an hindex of 11, co-authored 22 publications receiving 2737 citations. Previous affiliations of Dmitry Ter-Ovanesyan include Broad Institute & Harvard University.
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Journal ArticleDOI
Highly efficient Cas9-mediated transcriptional programming
Alejandro Chavez,Jonathan Scheiman,Suhani Vora,Benjamin W. Pruitt,Marcelle Tuttle,Eswar Prasad Ramachandran Iyer,Shuailiang Lin,Samira Kiani,Christopher D. Guzman,Daniel J. Wiegand,Dmitry Ter-Ovanesyan,Jonathan L. Braff,Noah Davidsohn,Benjamin E. Housden,Norbert Perrimon,Ron Weiss,John Aach,James J. Collins,George M. Church +18 more
TL;DR: An improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9 is described and demonstrated in activating endogenous coding and noncoding genes and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).
Journal ArticleDOI
Perturbation of m6A Writers Reveals Two Distinct Classes of mRNA Methylation at Internal and 5′ Sites
Schraga Schwartz,Maxwell R. Mumbach,Marko Jovanovic,Timothy C. Wang,Timothy C. Wang,Karolina Maciag,Karolina Maciag,G. Guy Bushkin,Philipp Mertins,Dmitry Ter-Ovanesyan,Naomi Habib,Davide Cacchiarelli,Davide Cacchiarelli,Neville E. Sanjana,Elizaveta Freinkman,Michael E. Pacold,Michael E. Pacold,Rahul Satija,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Nir Hacohen,Nir Hacohen,Feng Zhang,Feng Zhang,Feng Zhang,Steven A. Carr,Eric S. Lander,Eric S. Lander,Eric S. Lander,Aviv Regev,Aviv Regev,Aviv Regev +31 more
TL;DR: A dense network of proteins interacting with METTL3, a component of the methyltransferase complex, is identified, and it is shown that three of them (WTAP, METTL14, and KIAA1429) are required for methylation.
Journal ArticleDOI
Comparison of Cas9 activators in multiple species
Alejandro Chavez,Alejandro Chavez,Marcelle Tuttle,Benjamin W. Pruitt,Ben Ewen-Campen,Raj Chari,Dmitry Ter-Ovanesyan,Dmitry Ter-Ovanesyan,Sabina J Haque,Ryan J Cecchi,Emma J. K. Kowal,Joanna Buchthal,Benjamin E. Housden,Norbert Perrimon,Norbert Perrimon,James J. Collins,George M. Church,George M. Church +17 more
TL;DR: This work compares Cas9 activator systems and examines their ability to induce robust gene expression in several human, mouse, and fly cell lines, and assesses the role of cooperativity in maximizing gene expression.
Journal ArticleDOI
Cas9 gRNA engineering for genome editing, activation and repression
Samira Kiani,Alejandro Chavez,Alejandro Chavez,Marcelle Tuttle,Richard N. Hall,Raj Chari,Dmitry Ter-Ovanesyan,Dmitry Ter-Ovanesyan,Jason Qian,Jason Qian,Benjamin W. Pruitt,Jacob Beal,Suhani Vora,Suhani Vora,Suhani Vora,Joanna Buchthal,Emma J. K. Kowal,Mohammad R. Ebrahimkhani,James J. Collins,Ron Weiss,George M. Church,George M. Church +21 more
TL;DR: In this paper, the length of Cas9-associated guide RNA (gRNA) was altered to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein.
Highly efficient Cas9-mediated transcriptional programming
Alejandro Chavez,Jonathan Scheiman,Marcelle Tuttle,Shuailiang Lin,Samira Kiani,Dmitry Ter-Ovanesyan,Noah Davidsohn,Norbert Perrimon,Ron Weiss,John Aach,Suhani Vora,Benjamin W. Pruitt,Eswar Prasad Ramachandran Iyer,Christopher D. Guzman,Daniel J. Wiegand,Jonathan L. Braff,Benjamin E. Housden,James J. Collins,George M. Church +18 more
TL;DR: In this article, the authors describe the development of an improved transcriptional regulator through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to Cas9 and demonstrate its utility in activating expression of endogenous coding and non-coding genes, targeting several genes simultaneously and stimulating neuronal differentiation of induced pluripotent stem cells (iPSCs).