J
Jonathan L. Braff
Researcher at Wyss Institute for Biologically Inspired Engineering
Publications - 7
Citations - 1663
Jonathan L. Braff is an academic researcher from Wyss Institute for Biologically Inspired Engineering. The author has contributed to research in topics: Induced pluripotent stem cell & Cas9. The author has an hindex of 4, co-authored 7 publications receiving 1189 citations.
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Journal ArticleDOI
Highly efficient Cas9-mediated transcriptional programming
Alejandro Chavez,Jonathan Scheiman,Suhani Vora,Benjamin W. Pruitt,Marcelle Tuttle,Eswar Prasad Ramachandran Iyer,Shuailiang Lin,Samira Kiani,Christopher D. Guzman,Daniel J. Wiegand,Dmitry Ter-Ovanesyan,Jonathan L. Braff,Noah Davidsohn,Benjamin E. Housden,Norbert Perrimon,Ron Weiss,John Aach,James J. Collins,George M. Church +18 more
TL;DR: An improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9 is described and demonstrated in activating endogenous coding and noncoding genes and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).
Highly efficient Cas9-mediated transcriptional programming
Alejandro Chavez,Jonathan Scheiman,Marcelle Tuttle,Shuailiang Lin,Samira Kiani,Dmitry Ter-Ovanesyan,Noah Davidsohn,Norbert Perrimon,Ron Weiss,John Aach,Suhani Vora,Benjamin W. Pruitt,Eswar Prasad Ramachandran Iyer,Christopher D. Guzman,Daniel J. Wiegand,Jonathan L. Braff,Benjamin E. Housden,James J. Collins,George M. Church +18 more
TL;DR: In this article, the authors describe the development of an improved transcriptional regulator through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to Cas9 and demonstrate its utility in activating expression of endogenous coding and non-coding genes, targeting several genes simultaneously and stimulating neuronal differentiation of induced pluripotent stem cells (iPSCs).
Posted ContentDOI
Highly-efficient Cas9-mediated transcriptional programming
Alejandro Chavez,Jonathan Scheiman,Suhani Vora,Benjamin W. Pruitt,Marcelle Tuttle,Eswar Prasad Ramachandran Iyer,Samira Kiani,Christopher D. Guzman,Daniel J. Wiegand,Dmitry Ter-Ovanesyan,Jonathan L. Braff,Noah Davidsohn,Ron Weiss,John Aach,James J. Collins,George M. Church +15 more
TL;DR: The development of an improved transcriptional regulator through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to Cas9 is described and its utility in activating expression of endogenous coding and non-coding genes, targeting several genes simultaneously and stimulating neuronal differentiation of induced pluripotent stem cells (iPSCs).
Journal ArticleDOI
Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics.
Stephanie J. Yaung,Stephanie J. Yaung,Stephanie J. Yaung,Luxue Deng,Ning Li,Jonathan L. Braff,George M. Church,George M. Church,Lynn Bry,Harris H. Wang,Harris H. Wang,Georg K. Gerber +11 more
TL;DR: Temporal FUnctional Metagenomics sequencing (TFUMseq) as mentioned in this paper uses metagenomic DNA to construct large-scale heterologous expression libraries that are tracked over time by deep sequencing and computational methods.
Journal ArticleDOI
Characterization of Cas9–Guide RNA Orthologs
Jonathan L. Braff,Stephanie J. Yaung,Stephanie J. Yaung,Stephanie J. Yaung,Kevin M. Esvelt,George M. Church,George M. Church +6 more
TL;DR: The value of orthogonal Cas9-gRNA pairings that allow for concurrent activation and editing at independent target sites in the same cell are described and considerations for the desired specificity inCas9-coupled functions are discussed.