Showing papers by "Christopher J. Frederickson published in 2010"
TL;DR: It is found that for PC-12, HeLa and HT-29 cell lines as well as primary cultures of cardiac myocytes and neurons in vitro in differing media, approximately 5 nmol/L free zinc produced apparently healthy cells, but 20-fold higher or lower concentrations were usually harmful as judged by multiple criteria.
Abstract: The zinc(II) ion has recently been implicated in a number of novel functions and pathologies in loci as diverse as the brain, retina, small intestine, prostate, heart, pancreas, and immune system. ...
205 citations
Patent•
08 Oct 2010
TL;DR: In this paper, a light source designed to emit an excitatory light at a first known wavelength that activates a fluorescent emission having a second and different wavelength by at least one selected fluorescent reporter probe that generates a different fluorescent response when bound to zinc ion.
Abstract: The present invention provides apparatuses and methods for determining zinc ion concentration in a fluid sample. Some apparatuses of the invention include (i) a light source designed to emit an excitatory light at a first known wavelength that activates a fluorescent emission having a second and different wavelength by at least one selected fluorescent reporter probe that generates a different fluorescent response when bound to zinc ion; (ii) at least one sample-holding component adapted to hold a liquid sample in a pathway of the excitatory light; (iii) a photodetector for measuring fluorescent emission intensity generated by fluorescent reporter probe bound to zinc ion within the liquid sample; and (iv) electronic means for creating a data signal that correlates with fluorescent emission intensity generated by fluorescent reporter probe bound to zinc ion in the liquid sample to the free zinc ion concentration. Such apparatuses and methods can be used to determine the presence of or the risk of having prostate cancer in a subject.