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Rebecca A. Bozym
Researcher at University of Maryland, Baltimore
Publications - 10
Citations - 593
Rebecca A. Bozym is an academic researcher from University of Maryland, Baltimore. The author has contributed to research in topics: Zinc & Carbonic anhydrase. The author has an hindex of 6, co-authored 10 publications receiving 545 citations.
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Journal ArticleDOI
Measuring Picomolar Intracellular Exchangeable Zinc in PC-12 Cells Using a Ratiometric Fluorescence Biosensor
TL;DR: This work quantitatively image intracellular exchangeable zinc in an ordinary resting cell culture line (PC-12), using an excitation ratiometric fluorescent biosensor based on carbonic anhydrase (CA), and indicates that the resting concentration is approximately 5-10 pM in cytoplasm and nucleus.
Journal ArticleDOI
Free zinc ions outside a narrow concentration range are toxic to a variety of cells in vitro
Rebecca A. Bozym,Fabrice Chimienti,Leonard J. Giblin,Gunter W Gross,Irina Korichneva,Yuan Li,Yuan Li,Sarah Libert,Wolfgang Maret,Maryam Parviz,Christopher J. Frederickson,Richard B. Thompson +11 more
TL;DR: It is found that for PC-12, HeLa and HT-29 cell lines as well as primary cultures of cardiac myocytes and neurons in vitro in differing media, approximately 5 nmol/L free zinc produced apparently healthy cells, but 20-fold higher or lower concentrations were usually harmful as judged by multiple criteria.
Journal ArticleDOI
Quantitative Imaging of Mitochondrial and Cytosolic Free Zinc Levels in an in vitro Model of Ischemia/Reperfusion
Bryan J. McCranor,Rebecca A. Bozym,Michele Vitolo,Michele Vitolo,Carol A. Fierke,Linda L. Bambrick,Brian M. Polster,Gary Fiskum,Richard B. Thompson +8 more
TL;DR: An excitation ratiometric fluorescence biosensor based on human carbonic anhydrase II variants expressed in the mitochondrial matrix, permitting free zinc levels to be quantitatively imaged therein, is described.
Book ChapterDOI
Determination of zinc using carbonic anhydrase-based fluorescence biosensors.
Rebecca A. Bozym,Tamiika K. Hurst,Nissa Westerberg,Andrea K. Stoddard,Carol A. Fierke,Christopher J. Frederickson,Richard B. Thompson +6 more
TL;DR: This chapter summarizes the use of carbonic anhydrase (CA)-based fluorescent indicators to determine free zinc in solution, in cells, and in subcellular organelles.
PatentDOI
Excitation ratiometric fluoroscent biosensor for zinc ion at picomolar levels
TL;DR: In this article, a highly selective and sensitive carbonic anhydrase-based method for measurement of zinc ion by an excitation ratiometric format based on resonance energy transfer is provided.