Organic dyes have been used as gain medium for lasers since the 1960s, long before the advent of today’s organic electronic devices. Organic gain materials are highly attractive for lasing due to their chemical tunability and large stimulated emission cross section. While the traditional dye laser has been largely replaced by solid-state lasers, a number of new and miniaturized organic lasers have emerged that hold great potential for lab-on-chip applications, biointegration, low-cost sensing and related areas, which benefit from the unique properties of organic gain materials. On the fundamental level, these include high exciton binding energy, low refractive index (compared to inorganic semiconductors), and ease of spectral and chemical tuning. On a technological level, mechanical flexibility and compatibility with simple processing techniques such as printing, roll-to-roll, self-assembly, and soft-lithography are most relevant. Here, the authors provide a comprehensive review of the developments in the...

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Organic Lasers: Recent Developments on Materials, Device Geometries, and Fabrication Techniques

Optical microcavities that confi ne light in high-Q resonance promise all of the capabilities required for a successful nextgeneration microsystem biodetection technology. Label-free detection down to single molecules as well as operation in aqueous environments can be integrated cost-effectively on microchips, together with other photonic components, as well as electronic ones. We provide a comprehensive review of the sensing mechanisms utilized in this emerging fi eld, their physics, engineering and material science aspects, and their application to nanoparticle analysis and biomolecular detection. We survey the most recent developments such as the use of mode splitting for self-referenced measurements, plasmonic nanoantennas for signal enhancements, the use of optical force for nanoparticle manipulation as well as the design of active devices for ultra-sensitive detection. Furthermore, we provide an outlook on the exciting capabilities of functionalized high-Q microcavities in the life sciences.

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Label-free detection with high-Q microcavities: a review of biosensing mechanisms for integrated devices.

The next-generation passive optical network stage 2 (NG-PON2) effort was initiated by the full service access network (FSAN) in 2011 to investigate on upcoming technologies enabling a bandwidth increase beyond 10 Gb/s in the optical access network. The FSAN meeting in April 2012 selected the time- and wavelength-division multiplexed passive optical network (TWDM-PON) as a primary solution to NG-PON2. In this paper, we summarize the TWDM-PON research in FSAN by reviewing the basics of TWDM-PON and presenting the world's first full-system 40 Gb/s TWDM-PON prototype. After introducing the TWDM-PON architecture, we explore TWDM-PON wavelength plan options to meet the NG-PON2 requirements. TWDM-PON key technologies and their respective level of development are further discussed to investigate its feasibility and availability. The first full-system 40 Gb/s TWDM-PON prototype is demonstrated to provide 40 Gb/s downstream and 10 Gb/s upstream bandwidth. This full prototype system offers 38 dB power budget and supports 20 km distance with a 1:512 split ratio. It coexists with commercially deployed Gigabit PON (G-PON) and 10 Gigabit PON (XG-PON) systems. The operator-vendor joint test results testify that TWDM-PON is achievable by the reuse and integration of commercial devices and components.

Time- and Wavelength-Division Multiplexed Passive Optical Network (TWDM-PON) for Next-Generation PON Stage 2 (NG-PON2)

The electronics surrounding us in our daily lives rely almost exclusively on electrons as the dominant charge carrier. In stark contrast, biological systems rarely use electrons but rather use ions and molecules of varying size. Due to the unique combination of both electronic and ionic/molecular conductivity in conducting and semiconducting organic polymers and small molecules, these materials have emerged in recent decades as excellent tools for translating signals between these two realms and, therefore, providing a means to effectively interface biology with conventional electronics—thus, the field of organic bioelectronics. Today, organic bioelectronics defines a generic platform with unprecedented biological recording and regulation tools and is maturing toward applications ranging from life sciences to the clinic. In this Review, we introduce the field, from its early breakthroughs to its current results and future challenges.

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Organic Bioelectronics: Bridging the Signaling Gap between Biology and Technology.

Optical sensors represent a vitally important class of analytical tools that have been used to provide chemical information ranging from analyte concentration and binding kinetics to microscopic imaging and molecular structure. Optical sensors utilize a variety of signal transduction pathways based on photonic attributes that include absorbance, transmission, fluorescence intensity, refractive index, polarization, and reflectivity. Within the broad classification of optical sensors, refractive index (RI) sensors, which include devices such as surface plasmon resonance instruments, interferometers, diffraction gratings, optical fibers, photonic crystals, and resonant microcavities, have emerged as promising technologies over the past two decades. These optical sensors based on the change in RI associated with analyte binding involve an impressive array of instrumentation that allows for label-free1 molecular sensing without the added complexity of fluorescent or enzymatic tags. By removing the requirement for labels, RI-based sensing allows for real-time and direct detection of molecular interactions at a dielectric interface. Though many manifestations of RI-based sensors have been proposed and demonstrated, high-quality factor (high-Q) optical sensors based on multi-pass photonic microstructures have recently emerged as an extremely promising, and perhaps the most sensitive, class of label-free sensors. Major advantages of many high-Q sensors include multiple-pass interactions between the propagating electromagnetic radiation and the respective analyte binding event, as well as the intrinsic chip-integration and wafer-scale fabrication that accompany many semiconductor-based sensing modalities.

High-Q optical sensors involve microstructures that confine light due to differences in RI between a micropatterned material and its surrounding. This confinement supports multi-pass light interactions based on either multiple reflections or many circumnavigations. In both cases, this results in an increased effective optical path length that improves the sensitivity of the device. The Q factor of a given device is a measure of the resonant photon lifetime within a microstructure (higher Q factor = longer lifetime), and therefore Q is directly correlated to the number of times a photon is recirculated and allowed to interact with the analyte.2 Light is confined by either total internal reflection at a core/cladding interface (microcavities) or by the spatially periodic modulation of materials with different RI properties (photonic crystals), and resultant high-Q sensors interact with their local environments via an evanescent optical field that extends from the sensor surface and decays exponentially with distance.3, 4 A more detailed treatment of microcavity technology involving whispering gallery mode (WGM) sensing will be presented in the following section. High-Q optical sensors, whether based on guided-mode optics or photonic crystal (PC) structures, support resonances at very specific wavelengths, and these resonances are responsive to changes in the effective RI at the device surface. For most microcavity sensors, the wavelengths of light transmitted between an adjacent waveguide or optical fiber and the cavity is attenuated at narrow resonant wavelengths that are a function of the RI at the microcavity surface; for most PC sensors, light is back-reflected only at precise resonance wavelengths. As the Q factor of a device increases, the photon lifetime increases, and the resonance wavelength peak becomes narrower. For both microcavity and PC sensors, the relative shift in resonance wavelengths is directly proportional to the effective RI sampled by the confined optical mode, which samples the dielectric interface via the evanescent wave extending from the sensor surface. Since most analytes, such as organic (bio)molecules in water or gases in air, have a greater dielectric permittivity (and thus higher RI) than the surrounding medium, their binding or association with the sensor surface leads to an increase in effective RI sampled by the optical mode.4 Though factors such as biological and spectroscopic noise often set the practical limit of detection for any sensor system, the narrow resonance wavelengths associated with high-Q cavities provide an opportunity to resolve tiny spectral shifts that accompany a very small number of analyte binding interactions. The impressive sensitivity of microcavity and PC devices to minute changes in the effective RI at the sensor surface is the basis for most of the recent applications of high-Q optical sensors.

The development of high-Q photonic devices has been tremendously enabled by recent advances in micro- and nanofabrication methods, and the application of these devices for chemical and biomolecular analysis has only come to fruition within the past decade. This review focuses on the most exciting research in this area over the period of 2009–2011, although enabling findings and developments that precede this range are also covered. Recent reviews have summarized advances that may include some treatment of high-Q sensors, but these reviews have been broadly focused on advances in label-free sensors in general,5–8 on applications of silicon photonics that include sensing among many others,9 or on a general treatment of optical devices for sensing that includes the devices of interest.10–13 Other excellent reviews are more narrowly focused and cover different aspects of high-Q technology, focusing specifically on ring resonator technology,14, 15 microsphere resonators,16 photonic crystals,4, 17 microfluidic integration with optical sensors,18, 19 and high-Q mechanical sensors.20 This review considers recent advances in high-Q and ultra-high-Q optical sensors for addressing fundamental challenges in measurement science, giving special attention to those techniques that demonstrate useful chemical or biomolecular measurement capabilities within relevant real-world matrices. Although not rigorously fitting within some strict definitions of high-Q devices, photonic crystal sensors are covered as they represent an exciting complementary technology that, in many ways, is more advanced at present than many high-Q microcavity sensor configurations. As this review is intended to target the broad community of practicing analytical chemists, particular focus is given to signal transduction mechanisms, surface chemistry, assay methodologies, and interesting new measurement applications, leaving detailed explanations of device optics and engineering to other, more topical reviews21, 22 and the collection of articles from the optics community cited herein.

Specifically, this review will briefly discuss the theoretical basis of high-Q optical sensing, including the multitude of sensor geometries within the category of multi-pass optical sensors. Recent advances in high-Q sensor surface chemistry, capture agent immobilization, assay design, and amplification techniques are covered, as well as interesting demonstrations of these technologies in impact areas such as quantitative detection, affinity profiling, multiplexed sensing, nanoparticle analysis, light manipulation, lasers, thermal sensing, and integrated detection techniques. Finally, we provide our own critical analysis of the field in general, offering thoughts on areas in which improvements are most needed to inform the future outlook and reach the goals of high-Q optical sensing.

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High-Q optical sensors for chemical and biological analysis.

The fabrication of visible wavelength vertically emitting distributed feedback (DFB) lasers with a subwavelength grating fabricated by a replica molding process and an active polymer layer printed by a horizontal dipping process is reported The combined techniques enable the organic DFB laser to be uniformly fabricated over large surface areas upon a flexible plastic substrate, with an approach that is compatible with roll-based manufacturing Using a fixed grating period and depth, DFB laser output wavelength is controlled over a 35 nm range through manipulation of the waveguide layer thickness, which is controlled by the speed of the horizontal dipping process We also demonstrate that the active area of the structure may be photolithographically patterned to create dense arrays of discrete DFB lasers

Large-area organic distributed feedback laser fabricated by nanoreplica molding and horizontal dipping

A dielectric nanorod structure is used to enhance the label-free detection sensitivity of a vertically-emitting distributed feedback laser biosensor (DFBLB). The device is comprised of a replica molded plastic grating that is subsequently coated with a dye-doped polymer layer and a TiO2 nanorod layer produced by the glancing angle deposition technique. The DFBLB emission wavelength is modulated by the adsorption of biomolecules, whose greater dielectric permittivity with respect to the surrounding liquid media will increase the laser wavelength in proportion to the density of surface-adsorbed biomaterial. The nanorod layer provides greater surface area than a solid dielectric thin film, resulting in the ability to incorporate a greater number of molecules. The detection of a monolayer of protein polymer poly (Lys, Phe) is used to demonstrate that a 90 nm TiO2 nanorod structure improves the detection sensitivity by a factor of 6.6 compared to an identical sensor with a nonporous TiO2 surface.

Distributed feedback laser biosensor incorporating a titanium dioxide nanorod surface

Utilizing a tunable photonic crystal resonant reflector as a mirror of an external cavity laser cavity, we demonstrate a new type of label-free optical biosensor that achieves a high quality factor through the process of stimulated emission, while at the same time providing high sensitivity and large dynamic range. The photonic crystal is fabricated inexpensively from plastic materials, and its resonant wavelength is tuned by adsorption of biomolecules on its surface. Gain for the lasing process is provided by a semiconductor optical amplifier, resulting in a simple detection instrument that operates by normally incident noncontact illumination of the photonic crystal and direct back-reflection into the amplifier. We demonstrate single-mode, biomolecule-induced tuning of the continuous-wave laser wavelength. Because the approach incorporates external optical gain that is separate from the transducer, the device represents a significant advance over previous passive optical resonator biosensors and laser-based biosensors.

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External cavity laser biosensor

A process that combines polymer nanoreplica molding with horizontal dipping was used to fabricate large area (~ 3 × 5 inch2) distributed feedback laser biosensors (DFBLB) on flexible plastic substrates, which were subsequently incorporated into standard format 96-well microplates. A room temperature nanoreplica molding process was used to create subwavelength periodic grating structures, while a horizontal dipping process was used to apply a ~ 300 nm, dye-doped polymer film. In this work, the DFBLB emission wavelength, used to characterize the device uniformity, demonstrated a coefficient of variation (CV) of 0.41% over the fabricated device area, representing a thickness standard deviation of only ~ 35 nm for the horizontal dipping process. The fabricated sensors were further characterized for sensitivity uniformity by measuring the bulk refractive index of the media exposed to the sensor surface and by measuring adsorption of biomolecular layers. An assay for detection of the cytokine Tumor Necrosis Factor-alpha (TNF-α) was used to demonstrate the operation of the sensor in the context of label-free detection of a disease biomarker. The demonstrated capability represents an important step towards roll-to-roll manufacturability for this biosensor that simultaneously incorporates high sensitivity with excellent wavelength shift resolution, and adaptability to the microplate format that is ubiquitous in pharmaceutical research.

Plastic-Based Distributed Feedback Laser Biosensors in Microplate Format

Combining the high sensitivity properties of surface plasmon resonance refractive index sensing with a tunable external cavity laser, we demonstrate a plasmonic external cavity laser (ECL) for high resolution refractometric sensing. The plasmonic ECL utilizes a plasmonic crystal with extraordinary optical transmission (EOT) as the wavelength-selective element, and achieves single mode lasing at the transmission peak of the EOT resonance. The plasmonic ECL refractometric sensor maintains the high sensitivity of a plasmonic crystal sensor, while simultaneously providing a narrow spectral linewidth through lasing emission, resulting in a record high figure of merit for refractometric sensing with an active or passive optical resonator. We demonstrate single-mode and continuous-wave operation of the electrically-pumped laser system, and show the ability to measure refractive index changes with a 3σ detection limit of 1.79 × 10−6 RIU. The demonstrated approach is a promising path towards label-free optical biosensing with enhanced signal-to-noise ratios for challenging applications in small molecule drug discovery and pathogen sensing.

Chun Ge

Papers

Large-area organic distributed feedback laser fabricated by nanoreplica molding and horizontal dipping

Distributed feedback laser biosensor incorporating a titanium dioxide nanorod surface

External cavity laser biosensor

Plastic-Based Distributed Feedback Laser Biosensors in Microplate Format

Plasmonic external cavity laser refractometric sensor