D
D. Lee Kristensen
Researcher at Massachusetts Institute of Technology
Publications - 3
Citations - 37
D. Lee Kristensen is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Gene & Pichia pastoris. The author has an hindex of 3, co-authored 3 publications receiving 20 citations.
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Journal ArticleDOI
Comparative genome-scale analysis of Pichia pastoris variants informs selection of an optimal base strain.
Joseph R. Brady,Charles A. Whittaker,Melody C. Tan,D. Lee Kristensen,Duanduan Ma,Neil C. Dalvie,Kerry R. Love,J. Christopher Love +7 more
TL;DR: In this article, a comparative assessment of the relative fitness of these hosts for recombinant protein expression was performed using genomic and transcriptomic analyses of Y-11430 (CBS7435), GS115, X-33, and eight other variants.
Journal ArticleDOI
Molecular Engineering Improves Antigen Quality and Enables Integrated Manufacturing of A Trivalent Subunit Vaccine Candidate for Rotavirus
Neil C. Dalvie,Joseph R. Brady,Laura E. Crowell,Mary Kate Tracey,Andrew M. Biedermann,Kawaljit Kaur,John M. Hickey,D. Lee Kristensen,Alexandra D. Bonnyman,Sergio A. Rodriguez-Aponte,Charles A. Whittaker,M. Bok,Celina G. Vega,Tarit Mukhopadhyay,Sangeeta B. Joshi,David B. Volkin,Viviana Parreño,Kerry R. Love,J. Christopher Love +18 more
TL;DR: This study demonstrates that molecular engineering of subunit antigens using advanced genomic methods can facilitate their manufacturing in continuous production, and has potential to lower the cost and volumetric requirements in manufacturing vaccines based on recombinant protein subunits.
Posted ContentDOI
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Neil C. Dalvie,Joseph R. Brady,Laura E. Crowell,Mary Kate Tracey,Andrew M. Biedermann,Kawaljit Kaur,John M. Hickey,D. Lee Kristensen,Alexandra D. Bonnyman,Sergio A. Rodriguez-Aponte,Charles A. Whittaker,M. Bok,Celina G. Vega,Tarit Mukhopadhyay,Sangeeta B. Joshi,David B. Volkin,Viviana Parreño,Kerry R. Love,J. Christopher Love +18 more
TL;DR: In this paper, the authors describe a holistic approach for the molecular design of recombinant protein antigens, considering both their manufacturability and antigenicity, informed by bioinformatic analyses such as RNA-seq, ribosome profiling, and sequence-based prediction tools.