D Werner

TL;DR: Positive charged nanoparticles prepared from diethylaminoethyl (DEAE)-dextran and polyhexylcyanoacrylate (PHCA) were evaluated as carriers for ODNs and an active uptake mechanism, such as endocytosis, is proposed for the internalization of the ODN-nanoparticle formulations.

TL;DR: Cationic copolymer nanoparticles containing 30% (w/w) methylaminoethyl-methacrylate (MMAEMC) were found to be optimal with regard to biocompatibility and carrier properties for hydrophilic anionic antisense oligonucleotides.

TL;DR: Results of the cell penetration and the antisense assay demonstrated that MMAEMA nanoparticles are promising carriers for oligonucleotide administration and adsorption to nanoparticles led to an increased cellular uptake of oligon nucleotides and to a significantly enhanced antisense efficacy of unmodified phosphodiester oligon DNA as well as phosphorothioates.

TL;DR: Positive charged copolymer nanoparticles prepared from aminoalkyl- and methylmethacrylates as well as aminoethylmethACrylamide and aminohexylmethicrylate copolymers exhibited a strong positively charged surface even at physiological pH and, therefore, are useful candidates for the adsorption of anionic drugs.

TL;DR: A novel synthesis of cholest-5-en-3 beta-yl-6-aminohexyl ether (AH-Chol) was used to prepare positively charged liposomes, which consisted of phospholipon 90H and the cationic cholesterol derivative in an equimolar ratio.