D
Dave Siak-Wei Ow
Researcher at Agency for Science, Technology and Research
Publications - 35
Citations - 792
Dave Siak-Wei Ow is an academic researcher from Agency for Science, Technology and Research. The author has contributed to research in topics: Gene & Gene expression. The author has an hindex of 13, co-authored 33 publications receiving 700 citations. Previous affiliations of Dave Siak-Wei Ow include National University of Singapore.
Papers
More filters
Journal ArticleDOI
Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria.
TL;DR: QPCR was found to show good reproducibility and high sensitivity in detecting a two fold difference in template concentration, and a wide linear dynamic range covering 0.5 pg to 50 ng of DNA.
Journal ArticleDOI
Global transcriptional analysis of metabolic burden due to plasmid maintenance in Escherichia coli DH5α during batch fermentation
TL;DR: It is suggested that plasmid maintenance alone perturbs global gene regulation, and leads to significant changes in central metabolic pathways in the host.
Journal ArticleDOI
Sonochemistry and sonoluminescence in microfluidics
Tandiono,Siew-Wan Ohl,Dave Siak-Wei Ow,Evert Klaseboer,Victor V.T. Wong,Rainer Dumke,Claus-Dieter Ohl +6 more
TL;DR: This work presents a realization of sonoluminescence and sonochemistry created from bubbles confined within a narrow channel of polydimethylsiloxane-based microfluidic devices and finds the formation of OH radicals and the emission of light during bubble collapse.
Journal ArticleDOI
Creation of cavitation activity in a microfluidic device through acoustically driven capillary waves.
Tandiono,Siew-Wan Ohl,Dave Siak-Wei Ow,Evert Klaseboer,Victor V.T. Wong,Andrea Camattari,Claus-Dieter Ohl +6 more
TL;DR: It is found that strong forcing leads to the excitation of nonlinear surface waves when gas-liquid interfaces are present in the microfluidic channels and nuclei leading to intense inertial cavitation are generated by the entrapment of gas pockets at those interfaces.
Journal ArticleDOI
Sonolysis of Escherichia coli and Pichia pastoris in microfluidics
Tandiono Tandiono,Dave Siak-Wei Ow,Leonie Driessen,Cara Sze-Hui Chin,Evert Klaseboer,Andre Choo,Siew-Wan Ohl,Claus-Dieter Ohl +7 more
TL;DR: An efficient ultrasound based technique for lysing Escherichia coli and Pichia pastoris with oscillating cavitation bubbles in an integrated microfluidic system and qRT-PCR analysis show that functionality of GFP and genomic DNA for downstream analytical assays is maintained.