Showing papers by "David E. Cliffel published in 2003"
TL;DR: The Cytosensor™ microphysiometer has been modified by incorporating several platinum electrodes into the plunger head toMeasurement of two parameters, oxygen and acidification, enables investigation of the aerobic and anaerobic metabolic consequences of these agents.
50 citations
Patent•
27 Aug 2003TL;DR: In this article, a bioreactor for cultivating living cells in a liquid medium is presented. But the authors do not specify the type of cells to be harvested and only one predetermined type of cell is allowed to be transferred between the first sub-chamber and the second subchamber.
Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate (140) having a first surface (140a) and an opposite second surface (140b), defining a chamber(101) therebetween for receiving cells and the liquid medium. The bioreactor further has a barrier (104) dividing the chamber (101) into a first subchamber (102) and a second subchamber (103), wherein the barrier (104) has a porosity to allow the first subchamber 102) and the second subchamber (103) in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber (102) and the second subchamber (103) in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber (102) and the second subchamber (103).
40 citations
TL;DR: In this article, two planar boron-doped diamond electrodes were achieved by plasma enhanced chemical vapor deposition (PECVD) using in situ gas phase doping method.
Abstract: The unique electrochemical properties of diamond such as a large working potential window, low background current and prolonged stability make it attractive for applications in electroanalysis. High quality and conductive diamond films are known to exhibit active voltammetric response without the need for surface pretreatment. This paper reports on the design, fabrication and characterization of CVD diamond film for electrochemical sensing. Two types of planar boron-doped diamond electrodes were achieved by plasma enhanced chemical vapor deposition (PECVD) using in situ gas phase doping method. The first utilizes the “as grown” diamond surface with randomly microstructured topology as a planar diamond electrode. The second utilizes a micropatterning technique to produce a well-defined pyramidal diamond tips array with good uniformity control. The fabrication process for the electrodes is described. The diamond microelectrodes were evaluated electrochemically for the detection of ferrocyanide, Fe(CN) 6 4− , using cyclic voltammetry. The results suggest that diamond electrodes can be used in electrochemical sensing application.
35 citations
Patent•
27 Aug 2003TL;DR: In this article, a bioreactor for growing living cells in a liquid medium is described, where the flow of liquid is controlled so as to provide a known shear force to the layer of cells.
Abstract: A bioreactor (1100) for cultivating living cells in a liquid medium. (See Figure 2A1) In one embodiment of the present invention, the bioreactor (1100) includes a first substrate (1124) having a first surface (1124a), an opposite second surface (1124b) and edges. The bioreactor (1100) further includes a second substrate (1121) having a first surface (1121a) and an opposite second surface (1121b), defining a cavity (1121c) with a bottom surface (1121d), where the bottom surface (1121d) is located therebetween the first surface (1121a) and the second surface (1121b). The first surface (1121a) of the first substrate (1124) is received by the second surface (1121b ) of the second substrate (1121) to cover the cavity (1121c) so as to form a channel (1101) for receiving cells and a liquid medium. In forming the bioreactor, the channel (1101) is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel (1101). The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel (1101).
33 citations
Patent•
27 Aug 2003TL;DR: In this paper, a bioreactor for growing living cells in a liquid medium is described, where each of the plurality of chambers is adapted for receiving cells in liquid medium and formed with an open end (262), an opposite closed end (264) and side walls, the open end and the closed end defining a depth, d, therebetween for the corresponding chamber (206), the sidewalls defining a width, w, there between for the same or different from each other, as such formed, each chamber is in fluid communication with the channel (202) through the open
Abstract: A bioreactor (200) for cultivating living cells in a liquid medium. In one embodiment, the bioreactor (200) has a substrate (230) having a first surface and an opposite second surface, defining a channel (202) therein, and a plurality of chambers (204) formed in the substrate, wherein each of the plurality of chambers (204) is adapted for receiving cells in a liquid medium and formed with an open end (262), an opposite closed end (264) and side walls, the open end (262) and the closed end (264)defining a depth, d, therebetween for the corresponding chamber (206), the sidewalls defining a width, w, therebetween for the corresponding chamber (206). As such formed, each chamber (206) is in fluid communication with the channel (202) through the open end of the chamber (206), and at least two of the plurality of chambers (206) have depths or widths the same or different from each other.
14 citations