D
David E. Wolf
Researcher at Worcester Foundation for Biomedical Research
Publications - 46
Citations - 1575
David E. Wolf is an academic researcher from Worcester Foundation for Biomedical Research. The author has contributed to research in topics: Membrane & Sperm. The author has an hindex of 22, co-authored 46 publications receiving 1538 citations.
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Journal ArticleDOI
Selectivity of fluorescent lipid analogs for lipid domains
TL;DR: The phase partition preferences of the even chain length diacyl-3'3'-indocarbo-cyanine iodides incorporated in disaturated lecithin (PC) vesicles are examined and the pattern of preference is identical with that observed in dimyristoyl-PC, only shifted to longer chainlength diI's by two carbons.
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Determination of the transbilayer distribution of fluorescent lipid analogues by nonradiative fluorescence resonance energy transfer.
TL;DR: In this paper, the authors measured the nonradiative fluorescence resonance energy transfer between 7-nitro-2,1,3-benzoxadiazol-4-yl (NBD) labeled lipids (amine labeled phosphatidylethanolamine or acyl chain labeled pithylcholine) and rhodamine-labeled lipids in large unilamellar dioleoylphosphatidyl choline vesicles.
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Interaction with TrkA immobilizes gp75 in the high affinity nerve growth factor receptor complex.
David E. Wolf,Christine A. McKinnon,Marie-Claire Daou,Robert M. Stephens,David L. Kaplan,Alonzo H. Ross +5 more
TL;DR: Analysis of gp75 diffusion coefficients indicates that mutated gp75 and TrkA molecules may form a complex, even in the absence of the ability to bind NGF with high affinity, which paralleling the requirements of high affinity binding of NGF.
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Determination of the sidedness of carbocyanine dye labeling of membranes.
TL;DR: It is found that 2,4,6-trinitrobenzenesulfonate (TNBS) is an effective quencher of the fluorescence of the 1,1'-dialkyl-3,3, 3',3'-tetramethylindocarbocyanines (CNdiI's).
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Lipid domains in the ram sperm plasma membrane demonstrated by differential scanning calorimetry
TL;DR: In this article, the authors used differential scanning calorimetry to show that the anterior region of the head of a ram sperm exhibits at least two major endothermic transitions, one centered at approximately 26 degrees C and one centred at approximately 60 degrees C, consistent with gel-to-fluid transitions in model membranes.