Showing papers by "David Eisenberg published in 1981"
TL;DR: The determination of the crystal structure of tetrameric melittin at 2.8-A resolution by the method of multiple isomorphous replacement, followed by partial atomic refinement, suggests that theMelittin monomer contains two alpha-helical regions separated by a non-alpha-helicals segment at residues 11 and 12.
304 citations
TL;DR: It is shown that cell lines producing lgG likewise contain two mRNA species for immunoglobulin γ chains, and the flanking DNA sequences show a patchwork pattern of homology between genes that suggests a checkered evolutionary history.
79 citations
TL;DR: In this paper, the ribulose bisphosphate (RuBP) carboxylase molecule was characterized by point group symmetry D4 (422, see Figure 1 below).
Abstract: From studies of three crystal forms of ribulose bisphosphate (RuBP) carboxylase from Nicotiana tabacum (called I, II, and III), we have determined the subunit organization of RuBP carboxylase in increasing detail. Combined x-ray diffraction and electron microscope data from these crystals show that there must be some multiple of eight polypeptide chains in the molecule and that the polypeptides are arranged around a fourfold axis of symmetry. At low resolution the eight copies of each polypeptide are equivalent. In more formal terms, the RuBP carboxylase molecule is characterized by point group symmetry D4 (422, see Figure 1 below). The molecule has a square cross section, about 11 nm on an edge, and a cylindrical channel about 2 nm in diameter which runs along the fourfold axis perpendicular to the square cross section. Four large sub-units are arranged in a ring perpendicular to the fourfold axis, and two such rings are eclipsed, forming a two-level structure that extends about 10 nm along the fourfold axis.
9 citations
TL;DR: Bur and Eisenberg as mentioned in this paper described a procedure for the isolation of glutamine synthetase and the protein product of the groE gene (p groE ) by polyethyleneimine precipitation and affinity chromatography on a Blue Dextran column.
4 citations