Showing papers in "Cell in 1981"

TL;DR: Three transformed lines of simian cells were established and found to contain T antigen; retain complete permissiveness for lytic growth of SV40; support the replication of tsA209 virus at 40 degrees C; and support the replicate of pure populations of SV 40 mutants with deletions in the early region.

TL;DR: The mouse mitochondrial DNA genome is highly homologous in overall sequence and in gene organization to human mitochondrial DNA, with the descending order of conserved regions being tRNA genes; origin of light-strand replication; r RNA genes; knownprotein-coding genes; unidentified protein-c coding genes; displacement-loop region.

TL;DR: The activation of genes by specific enhancer elements seems to be a widespread mechanism that may be used for the regulation of gene expression and defines a class of DNA elements with a mode of action that has not been heretofore described.

TL;DR: It is suggested that osteonectin is a tissue-specific protein, linking the bone mineral and collagen phases, perhaps initiating active mineralization in normal skeletal tissue.

TL;DR: The variable portion of an immunoglobulin heavy chain gene is assembled from at least three discontinuous segments of DNA, the V, D and J regions, and the large number of human J region genes and, hence, their greater potential for generating diversity as compared to the that of the mouse J regions appears to result from recent genetic duplications.

TL;DR: It is proposed that Tetrahymena pre-rRNA splicing occurs by a phosphoester transferase mechanism where the guanosine cofactor provides the free 3' hydroxyl necessary to initiate a series of three transfers that results in splicing of the pre- rRNA and cyclization of the excised IVS.

TL;DR: It is proposed that the initial event in the secretion of proteins across membranes and their insertion into membranes is the spontaneous penetration of the hydrophobic portion of the bilayer by a helical hairpin.

TL;DR: Characterization of haploid double- sec -mutant strains, with regard to the structure of the accumulated invertase and the morphology of the exaggerated organelles, allows assessment of the order in which the gene products are required, the sequence of invert enzyme maturation steps and a pathway of secretory organelle pathways.

TL;DR: The fetal calf serum supplement of cell-culture medium contains enough of the vitamin to affect the differentiation of cultured keratinocytes derived from epidermis and from other stratified squamous epithelia.

TL;DR: It is shown that virions and purified viral cores contain a unique endonuclease that cleaves RNAs containing a 5' methylated cap structure preferentially at purine residues 10 to 14 nucleotides from the cap, generating fragments with 3'-terminal hydroxyl groups.

TL;DR: A plasmid containing the structural gene for thymidine kinase from herpes simplex virus fused to the promoter/regulatory region of the mouse metallothionein-I gene was injected into the pronucleus of fertilized one-cell mouse eggs; the eggs were subsequently reimplanted into the oviducts of pseudopregnant mice.

TL;DR: Three effects of deletion mutations on polyadenylation of late SV40 mRNAs are observed, including a 16 bp deletion that includes the AAUAAA sequence that prevents poly(A) addition.

TL;DR: Increased phosphorylation of tyrosine is the most rapid response of cells to EGF known, and may play an important role in the biological effects of EGF.

TL;DR: It is suggested that all vertebrate and invertebrate intermediate filament proteins share a common antigenic determinant and the possibility that all intermediate filaments contain a 66,000 molecular weight protein.

TL;DR: It is concluded that the differences between the genes resulted from somatic mutation and that the two hybridomas derived their VH regions from the same germ line gene.

TL;DR: It is demonstrated that BP antigen is synthesized by epidermal cells in culture, different patients with BP have antibodies against the same protein, and BP antigen can be identified on SDS-PAGE as a high molecular weight protein consisting of disulfide-linked chains of approximate molecular weight 220 kd.

TL;DR: Evidence is provided that in normal fibroblasts about 50% of surface LDL receptors absence of LDL can be induced to recycle by the presence of LDL; and monensin interrupts this recycling by preventing the receptor from returning to the surface, thereby causing the receptors to accumulate within the cell.

TL;DR: The map location of tumor-derived RNA transcribed from the T-DNA, the role of phytohormones in crown gall tumorigenesis and the eventual use of the Ti plasmid as a vehicle for introducing genes of choice into the genomes of higher plants are discussed.

TL;DR: The discoveries are reviewed that elucidate how the cytoskeleton of the human erythrocyte membrane is linked to the membrane and make it possible to visualize the molecular interactions among the cytOSkeletal components.

TL;DR: The modification of vinculin by p60src may be responsible in part for the disruption of the microfilament organization and for the changes in cell shape and adhesiveness which accompany transformation by Rous sarcoma virus.

TL;DR: It was found that early transcription could be detected in mutant-infected cells if the virus was added to cells in which protein synthesis was inhibited prior to infection, and it was proposed that the role of the E1A gene product in the activation of early viral transcription is to inactivate a cellular factor that prevents transcription from the early viral promoters.

TL;DR: It is suggested that the wild-type unc-86 and lin-4 genes act to modify latent reiterative cell lineages, which are revealed when the activity of one of these genes is eliminated.

TL;DR: A rapid and simple method for studying the transcription of cloned eucaryotic genes is developed, which involves transfecting SV40-transformed monkey cell lines (COS cells) with derivatives of the plasmid pBR322 that contain the SV40 viral replication origin but lack regions necessary for viral transcription (SV-ORI vectors).

TL;DR: It appears that the sequences between 16 and 32 nucleotides upstream from the 5' terminus of the tk gene are required to specify the exact start site of transcription, however, control of both the efficiency of transcription and rough specification of the position of initiation depends on sequences 40--100 nucleotide upstream fromthe tk structural gene.

TL;DR: A temperature-sensitive conditional lethal mutant is isolated that accumulates the precursor of the maltose-binding protein in the cytoplasm, and also accumulates precursors of alkaline phosphatase, lambda receptor protein and the ompF gene gene product.

TL;DR: It is suggested that the foundation of the trophectoderm and inner cell mass lineages lineages may occur by a process of differential inheritance, which supports the recently proposed polarization hypothesis.

TL;DR: The results show that viral gene products are not required for maintenance of neoplastic transformation and suggest that in lymphoid tumors, an ALV provirus is integrated adjacent to a specific cellular gene, and the insertion of the viral promoter adjacent to this gene results in its enhanced expression, leading to neoplasia.

TL;DR: It appears that transcriptional events are primarily responsible for the synthesis of these, and perhaps most, tissue-specific moderately abundant mRNAs.

TL;DR: It is now thought that at each of the recognized loci in the H-2 complex up to a hundred alleles may exist in the population and a high degree of polymorphism has been demonstrated.