D
David S. Wunschel
Researcher at Pacific Northwest National Laboratory
Publications - 56
Citations - 1787
David S. Wunschel is an academic researcher from Pacific Northwest National Laboratory. The author has contributed to research in topics: Mass spectrometry & Bacillus anthracis. The author has an hindex of 19, co-authored 55 publications receiving 1706 citations. Previous affiliations of David S. Wunschel include Environmental Molecular Sciences Laboratory & University of South Carolina.
Papers
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Journal ArticleDOI
Isolation of a high-affinity functional protein complex between OmcA and MtrC: Two outer membrane decaheme c-type cytochromes of Shewanella oneidensis MR-1.
Liang Shi,Baowei Chen,Zheming Wang,Dwayne A. Elias,M. Uljana Mayer,Yuri A. Gorby,Shuison Ni,Brian H. Lower,David W. Kennedy,David S. Wunschel,Heather M. Mottaz,Matthew J. Marshall,Eric A. Hill,Alexander S. Beliaev,John M. Zachara,James K. Fredrickson,Thomas C. Squier +16 more
TL;DR: It is demonstrated that OmcA and MtrC can interact directly with each other to form a stable complex that is consistent with their role in the electron transport pathway of S. oneidensis MR-1.
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Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry.
TL;DR: This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive identification with MALDI-TOF MS is still possible with the protocol established at the Pacific Northwest National Laboratory.
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Characterization of PCR products from bacilli using electrospray ionization FTICR mass spectrometry.
David C. Muddiman,David S. Wunschel,Chuanliang Liu,Ljiljana Paša-Tolić,Karen F. Fox,Alvin Fox,Gordon A. Anderson,Richard Smith +7 more
TL;DR: This constitutes the first report demonstrating the ionization and detection of PCR products by mass spectrometry with mass precision and accuracy for assignment of such modifications or substitutions.
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Discrimination Among the B. Cereus Group, in Comparison to B. Subtilis, by Structural Carbohydrate Profiles and Ribosomal RNA Spacer Region PCR
TL;DR: In this paper, the 16S / 23S rRNA spacer region was amplified with three major products: 270, 400, and 430 nucleotides, with the corresponding bands in the B. cereus group at 250, 430, and 480.
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Analysis of Double‐stranded Polymerase Chain Reaction Products from the Bacillus cereus Group by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
TL;DR: The analysis of polymerase chain reaction (PCR) products by electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR) has been achieved and the first report demonstrating the ionization and detection of a double-stranded PCR product by ESI-MS is demonstrated.