Showing papers by "Dean D. Erdman published in 2005"
TL;DR: Application of these assays for detection of adenoviruses and type-specific identification of AdV40 and AdV41 will be useful for identifying these viruses in environmental and clinical samples.
Abstract: A quantitative real-time TaqMan PCR assay for detection of human adenoviruses (HAdV) was developed using broadly reactive consensus primers and a TaqMan probe targeting a conserved region of the hexon gene. The TaqMan assay correctly identified 56 representative adenovirus prototype strains and field isolates from all six adenovirus species (A to F). Based on infectious units, the TaqMan assay was able to detect as few as 0.4 and 0.004 infectious units of adenovirus serotype 2 (AdV2) and AdV41, respectively, with results obtained in less than 90 min. Using genomic equivalents, the broadly reactive TaqMan assay was able to detect 5 copies of AdV40 (which had zero mismatches with the PCR primers and probe), 8 copies of AdV41, and 350 copies of AdV3 (which had the most mismatches [seven] of any adenovirus serotype tested). For specific detection and identification of F species serotypes AdV40 and AdV41, a second real-time PCR assay was developed using fluorescence resonance energy transfer (FRET) probes that target the adenovirus fiber gene. The FRET-based assay had a detection limit of 3 to 5 copies of AdV40 and AdV41 standard DNA and was able to distinguish between AdV40 and AdV41 based on melting curve analysis. Both the TaqMan and FRET PCR assays were quantitative over a wide range of virus titers. Application of these assays for detection of adenoviruses and type-specific identification of AdV40 and AdV41 will be useful for identifying these viruses in environmental and clinical samples.
253 citations
TL;DR: In six of these nine patients, the decline in FEV‐1 was sustained over a 1‐year period consistent with bronchiolitis obliterans syndrome (BOS), and community‐acquired respiratory viruses may be associated with the development of acute rejection and BOS.
238 citations
TL;DR: Monoclonal antibody MAb-8 was evaluated for detection of human metapneumovirus (HMPV) in shell vial centrifugation cultures (SVCC) and staining was optimal on day 2 postinoculation.
Abstract: Monoclonal antibody MAb-8 was evaluated for detection of human metapneumovirus (HMPV) in shell vial centrifugation cultures (SVCC). Detection of HMPV was similar in A549, HEp-2, and LLC-MK2 SVCC, and MAb-8 staining was optimal on day 2 postinoculation. Availability of SVCC for HMPV will be of significant benefit to clinical laboratories.
60 citations
TL;DR: This work is supported by the Ministry of Education, Culture, Sports, Science, and Technology of Japan and the Infectious Diseases Society of America.
Abstract: Financial support: Ministry of Education, Culture, Sports, Science, and Technology (grant-in-aid for exploratory research no. 14657179). Reprints and correspondence: Dr. Hideaki Kikuta, Dept. of Pediatrics, Hokkaido University Graduate School of Medicine, N15, W-7, Kita-ku, Sapporo 060-8638, Japan (hide-ki@med .hokudai.ac.jp). The Journal of Infectious Diseases 2005;192:351–2 2005 by the Infectious Diseases Society of America. All rights reserved. 0022-1899/2005/19202-0022$15.00
56 citations
TL;DR: This evaluation of adenovirus type 7 isolates collected in Iowa from 1992 to 2002 found that genome type Ad7d2 became increasingly prevalent and had supplanted all other Ad7 genome types by 2002.
Abstract: We evaluated 76 adenovirus type 7 (Ad7) isolates collected in Iowa from 1992 to 2002 and found that genome type Ad7d2 became increasingly prevalent. By 2002, it had supplanted all other Ad7 genome types. The association of Ad7d2 with severe illness and death calls for heightened public health concern.
22 citations