D
Denis F. Hochstrasser
Researcher at Geneva College
Publications - 303
Citations - 23622
Denis F. Hochstrasser is an academic researcher from Geneva College. The author has contributed to research in topics: Proteome & Gel electrophoresis. The author has an hindex of 80, co-authored 303 publications receiving 22841 citations. Previous affiliations of Denis F. Hochstrasser include Swiss Institute of Bioinformatics & Protein Sciences.
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Journal ArticleDOI
Progress with Proteome Projects: Why all Proteins Expressed by a Genome Should be Identified and How To Do It
Marc R. Wilkins,Jean-Charles Sanchez,Andrew A. Gooley,Ron D. Appel,Ian Humphery-Smith,Denis F. Hochstrasser,Keith L. Williams +6 more
TL;DR: The Progress with Proteome Projects: Why all Proteins Expressed by a Genome Should be Identified and How To Do It as discussed by the authors is an example of such a project.
Journal ArticleDOI
The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences
Bengt Bjellqvist,Graham J. Hughes,Christian Pasquali,Nicole Paquet,Florence Ravier,Jean-Charles Sanchez,Séverine Frutiger,Denis F. Hochstrasser +7 more
TL;DR: The advantages of being able to predict the position of a protein of known structure within a two‐dimensional gel are shown.
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From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Amino Acid Analysis
Marc R. Wilkins,Christian Pasquali,Ron D. Appel,Keli Ou,Olivier Golaz,Jean-Charles Sanchez,Jun X. Yan,Andrew A. Gooley,Graham J. Hughes,Ian Humphery-Smith,Keith L. Williams,Denis F. Hochstrasser +11 more
TL;DR: Single protein spots, from polyvinylidene difluoride blots of micropreparative E. coli 2-D gels, were rapidly and economically identified by matching their amino acid composition, estimated pI and molecular weight against all E. bacteria entries in the SWISS-PROT database.
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The dynamic range of protein expression: a challenge for proteomic research.
TL;DR: It is believed that most of the existing technology is capable of displaying many more proteins than is currently achievable by integrating existing and new techniques to prefractionate samples prior to 2‐DE display or analysis.
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Methods for increasing the resolution of two-dimensional protein electrophoresis.
Denis F. Hochstrasser,Michael G. Harrington,Anne-C. Hochstrasser,Mark J. Miller,Carl R. Merril +4 more
TL;DR: A two-dimensional gel elctrophoresis protocol has been developed which provides for a 1.5-to 3-fold increase in the resolution of proteins compared to other frequently used methods.