A fast-Fourier-transform method of topography and interferometry is proposed. By computer processing of a noncontour type of fringe pattern, automatic discrimination is achieved between elevation and depression of the object or wave-front form, which has not been possible by the fringe-contour-generation techniques. The method has advantages over moire topography and conventional fringe-contour interferometry in both accuracy and sensitivity. Unlike fringe-scanning techniques, the method is easy to apply because it uses no moving components.

Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry

From the Publisher: 
The accessible presentation of this book gives both a general view of the entire computer vision enterprise and also offers sufficient detail to be able to build useful applications. Users learn techniques that have proven to be useful by first-hand experience and a wide range of mathematical methods. A CD-ROM with every copy of the text contains source code for programming practice, color images, and illustrative movies. Comprehensive and up-to-date, this book includes essential topics that either reflect practical significance or are of theoretical importance. Topics are discussed in substantial and increasing depth. Application surveys describe numerous important application areas such as image based rendering and digital libraries. Many important algorithms broken down and illustrated in pseudo code. Appropriate for use by engineers as a comprehensive reference to the computer vision enterprise.

Computer vision : a modern approach = 计算机视觉 : 一种现代的方法

Digital holographic microscopy enables a quantitative phase contrast metrology that is suitable for the investigation of reflective surfaces as well as for the marker-free analysis of living cells. The digital holographic feature of (subsequent) numerical focus adjustment makes possible applications for multifocus imaging. An overview of digital holographic microscopy methods is described. Applications of digital holographic microscopy are demonstrated by results obtained from livings cells and engineered surfaces.

Digital holographic microscopy for live cell applications and technical inspection

The oral health surveys basic methods that we provide for you will be ultimate to give preference. This reading book is your chosen book to accompany you when in your free time, in your lonely. This kind of book can help you to heal the lonely and get or add the inspirations to be more inoperative. Yeah, book as the widow of the world can be very inspiring manners. As here, this book is also created by an inspiring author that can make influences of you to do more.

Oral Health Surveys: Basic Methods

The advent of biodegradable polymers has significantly influenced the development and rapid growth of various technologies in modern medicine. Biodegradable polymers are mainly used where the transient existence of materials is required and they find applications as sutures, scaffolds for tissue regeneration, tissue adhesives, hemostats, and transient barriers for tissue adhesion, as well as drug delivery systems. Each of these applications demands materials with unique physical, chemical, biological, and biomechanical properties to provide efficient therapy. Consequently, a wide range of degradable polymers, both natural and synthetic, have been investigated for these applications. Furthermore, recent advances in molecular and cellular biology, coupled with the development of novel biotechnological drugs, necessitate the modification of existing polymers or synthesis of novel polymers for specific applications. This review highlights various biodegradable polymeric materials currently investigated for use in two key medical applications: drug delivery and tissue engineering.

https://link.springer.com/content/pdf/10.1007%2Fb137240.pdf

Polymers as biomaterials for tissue engineering and controlled drug delivery

Digital holography enables a multifocus quantitative phase microscopy for the investigation of reflective surfaces and for marker-free live cell imaging. For digital holographic long-term investigations of living cells an automated (subsequent) robust and reliable numerical focus adjustment is of particular importance. Four numerical methods for the determination of the optimal focus position in the numerical reconstruction and propagation of the complex object waves of pure phase objects are characterized, compared, and adapted to the requirements of digital holographic microscopy. Results from investigations of an engineered surface and human pancreas tumor cells demonstrate the applicability of Fourier-weighting- and gradient-operator-based methods for robust and reliable automated subsequent numerical digital holographic focusing.

Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging

Digital holographic microscopy (DHM) enables a quantitative multifocus phase contrast imaging that has been found suitable for technical inspection and quantitative live cell imaging. The combination of DHM with fast and robust autofocus algorithms enables subsequent automated focus realignment by numerical propagation of the digital holographically reconstructed object wave. In combination with a calibrated optical imaging system, the obtained propagation data quantify axial displacements of the investigated sample. The evaluation of quantitative DHM phase contrast images also enables an effective determination of lateral cell displacements. Thus, 3-D displacement data are provided. Results from investigations on sedimenting red blood cells and HT-1080 fibrosarcoma cells in a collagen tissue model demonstrate that DHM enables marker-free automated quantitative dynamic 3-D cell tracking without mechanical focus adjustment.

https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-14/issue-01/014018/Automated-three-dimensional-tracking-of-living-cells-by-digital-holographic/10.1117/1.3080133.pdf

Automated three-dimensional tracking of living cells by digital holographic microscopy

Statement of problem. The insertion of prosthodontic restorations often leads to speech defects, most of which are transient but nevertheless a source of concern to the patient. For the dental practitioner, there are few guidelines on designing a prosthetic restoration with maximum phonetic success. Purpose. This study investigated the effect of different maxillary central incisor positions on phonetic patterns. Material and methods. The inclination angle of central incisor blocks in duplicate complete maxillary dentures was changed in a range of −30 degrees to +30 degrees from the original position (0 degrees). Test words and sentences were acoustically analyzed. Spectral parameters such as first peak, noise band (defined by upper and lower limits of the most intensively blackened frequency area), Fast Fourier Transformation power spectrum, energy spectrum, and time were investigated. Influences of oral stereognosis, hearing, and age as cofactors on /s/ articulation were also taken into account. Results. The change of incisor block angle in both directions usually caused a poorer execution of the /s/ sound. The labial angulation seemed to have a greater effect than the palatal angulation. The most significant changes were those in the noise band and energy spectrum. Conclusion. Immediate phonetic adaptation of prosthetic restorations in the maxillary incisor region can be achieved only if the original position of the natural teeth is transferred to the denture. Although these misarticulations are, in most cases, likely to disappear within a few weeks, they may persist and even lead to psychosocial problems. A better understanding of the causes of misarticulation and the limits of adaptation according to certain morphologic parameters of dentures is important. (J Prosthet Dent 2001;85:485-95.)

The influence of maxillary central incisor position in complete dentures on /s/ sound production.

Symmetry is a significant factor, among others, influencing the attractiveness of human faces. Hence its restoration is an essential task in oral and maxillofacial surgery. Until recently, most of the techniques to objectively quantify the facial asymmetry were based on the evaluation of two-dimensional data, i.e. photographs. These techniques are well-established in literature and have in common that several landmarks in the face are identified manually and put together in a formula to obtain a facial asymmetry value. The increasing availability of three-dimensional measuring systems offers the possibility to perform an automated 3D analysis of the facial symmetry based on a dense 3D point cloud of the facial surface. The results of this analysis are on the one hand a mirror plane, which meets best the partial symmetry of the face regarding the median sagittal plane, and on the other hand a 3D asymmetry index quantifying the overall facial asymmetry characteristics. In this paper three selected 2D analysis methods and one 3D analysis method are compared.

2D and 3D analysis methods of facial asymmetry in comparison.

The potential of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for quantification of cell culture quality was explored. Label-free QPI of detached single cells in suspension was performed by Michelson interferometer-based self-interference DHM. Two pancreatic tumor cell lines were chosen as cellular model and analyzed for refractive index, volume, and dry mass under varying culture conditions. Firstly, adequate cell numbers for reliable statistics were identified. Then, to characterize the performance and reproducibility of the method, we compared results from independently repeated measurements and quantified the cellular response to osmolality changes of the cell culture medium. Finally, it was demonstrated that the evaluation of QPI images allows the extraction of absolute cell parameters which are related to cell layer confluence states. In summary, the results show that QPI enables label-free imaging cytometry, which provides novel complementary integral biophysical data sets for sophisticated quantification of cell culture quality with minimized sample preparation. © 2017 International Society for Advancement of Cytometry.

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.a.23082

Dieter Dirksen

Papers

Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging

Automated three-dimensional tracking of living cells by digital holographic microscopy

The influence of maxillary central incisor position in complete dentures on /s/ sound production.

2D and 3D analysis methods of facial asymmetry in comparison.

Quantitative phase imaging for cell culture quality control.