D
Dieter Klein
Researcher at University of Veterinary Medicine Vienna
Publications - 71
Citations - 2993
Dieter Klein is an academic researcher from University of Veterinary Medicine Vienna. The author has contributed to research in topics: Feline immunodeficiency virus & Virus. The author has an hindex of 25, co-authored 71 publications receiving 2819 citations. Previous affiliations of Dieter Klein include University of Padua & University of Veterinary Science.
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Journal ArticleDOI
Quantification using real-time PCR technology: applications and limitations
TL;DR: The introduction of real-time PCR technology has significantly improved and simplified the quantification of nucleic acids, and this technology has become an invaluable tool for many scientists working in different disciplines.
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Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification.
TL;DR: It is demonstrated that minor mismatches in the primer or the probe region, decrease overall PCR efficiency but do not abolish the quantification, in contrast to major mismatches of three or four nucleotides, which lead to complete inhibition of the real‐time PCR detection.
Journal ArticleDOI
Transmission of small ruminant lentiviruses.
B.A. Blacklaws,E. Berriatua,Sigurbjörg Torsteinsdóttir,Neil J. Watt,D. de Andrés,Dieter Klein,Gordon D. Harkiss +6 more
TL;DR: Small ruminant lentiviruses (SRLV) are classical slow retroviruses causing chronic inflammatory disease in a variety of target organs, and have been detected in semen suggesting a potential source of transmission.
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Diagnostic tests for small ruminant lentiviruses.
D. de Andrés,Dieter Klein,Neil J. Watt,E. Berriatua,Sigurbjörg Torsteinsdóttir,B.A. Blacklaws,Gordon D. Harkiss +6 more
TL;DR: Recent developments in laboratory diagnostic methods and their use in field diagnosis suggest that a combination of ELISA and PCR might afford optimal detection of SRLV infection.
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Detection and quantification of the iap gene of Listeria monocytogenes and Listeria innocua by a new real-time quantitative PCR assay.
TL;DR: A real-time quantitative polymerase chain reaction (PCR) assay for direct detection and enumeration of Listersia monocytogenes and Listeria innocua was developed and applied to artificially contaminated milk samples, characterized by a wide dynamic range of quantification and a high sensitivity.