D
Donald C. Rio
Researcher at Cold Spring Harbor Laboratory
Publications - 28
Citations - 1569
Donald C. Rio is an academic researcher from Cold Spring Harbor Laboratory. The author has contributed to research in topics: RNA & Gel electrophoresis. The author has an hindex of 14, co-authored 28 publications receiving 1135 citations.
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Purification of RNA using TRIzol (TRI reagent).
TL;DR: TRIzol solubilization and extraction is a relatively recently developed general method for deproteinizing RNA, which is particularly advantageous in situations where cells or tissues are enriched for endogenous RNases or when separation of cytoplasmic RNA from nuclear RNA is impractical.
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Filter-binding assay for analysis of RNA-protein interactions.
TL;DR: This protocol requires a purified protein (or chromatographic fractions) of interest and (32)P-labeled RNA to perform the assay, and a 96-well dot-blot apparatus with low vacuum to trap the complexes on three membranes.
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Purification of RNA by SDS Solubilization and Phenol Extraction
TL;DR: This protocol describes a method for RNA purification by sodium dodecyl sulfate (SDS) solubilization and phenol extraction, which is of wide utility and is used routinely to deproteinize RNAs in biological material that has beensolubilized in SDS, an ionic detergent that dissolves membranes, disrupts protein-nucleic acid interactions, and inactivates ribonucleases.
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Preparation of Cytoplasmic and Nuclear RNA from Tissue Culture Cells
TL;DR: This protocol describes a method for separating nuclei from the cytoplasm that can be used for many tissue culture types, and is useful for cells grown in suspension or for adherent cells.
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Northern blots for small RNAs and microRNAs.
TL;DR: This protocol describes the detection of small RNAs by blot hybridization by electrophoretic transfer to positively charged nylon membranes, commonly used to detect small, U-rich spliceosomal small nuclear RNAs and miRNAs.