T
Timothy W. Nilsen
Researcher at Case Western Reserve University
Publications - 131
Citations - 9109
Timothy W. Nilsen is an academic researcher from Case Western Reserve University. The author has contributed to research in topics: RNA & RNA splicing. The author has an hindex of 42, co-authored 128 publications receiving 8225 citations.
Papers
More filters
Journal ArticleDOI
Expansion of the eukaryotic proteome by alternative splicing
TL;DR: It is now clear that the 'missing' information is in large part provided by alternative splicing, the process by which multiple different functional messenger RNAs, and therefore proteins, can be synthesized from a single gene.
Journal ArticleDOI
Purification of RNA using TRIzol (TRI reagent).
TL;DR: TRIzol solubilization and extraction is a relatively recently developed general method for deproteinizing RNA, which is particularly advantageous in situations where cells or tissues are enriched for endogenous RNases or when separation of cytoplasmic RNA from nuclear RNA is impractical.
Journal ArticleDOI
Mechanisms of microRNA-mediated gene regulation in animal cells
TL;DR: The evidence that there are multiple mechanisms of microRNA-mediated repression is described and the possible connections between these mechanisms are discussed.
Journal ArticleDOI
Functional recognition of the 3' splice site AG by the splicing factor U2AF35.
TL;DR: In this article, the authors used site-specific crosslinking to show that very early during spliceosome assembly U2AF35 directly contacts the 3' splice site, and explained why the AG dinucleotide is required for the first step of splicing for some but not all introns.
Journal ArticleDOI
The spliceosome: the most complex macromolecular machine in the cell?
TL;DR: Technical advances, including new affinity purification methods and improved mass spectrometry techniques, coupled with the completion of many genome sequences, have now permitted a number of proteomic analyses of purified spliceosomes, revealing that thespliceosome is composed of as many as 300 distinct proteins and five RNAs, making it among the most complex macromolecular machines known.