D
Doug Tischer
Researcher at University of Washington
Publications - 13
Citations - 938
Doug Tischer is an academic researcher from University of Washington. The author has contributed to research in topics: Biology & Protein design. The author has an hindex of 4, co-authored 8 publications receiving 366 citations. Previous affiliations of Doug Tischer include University of California, San Francisco.
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Journal ArticleDOI
Illuminating cell signalling with optogenetic tools
Doug Tischer,Orion D. Weiner +1 more
TL;DR: The optogenetic toolkit is moving beyond proof of concept to answering real biological questions, such as how cell signalling is regulated in space and time, that were difficult or impossible to address with previous tools.
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Robust deep learning based protein sequence design using ProteinMPNN
Justas Dauparas,Ivan Anishchenko,N. Bennett,Hua Bai,Robert J. Ragotte,Lukas F. Milles,Basile I. M. Wicky,Alexis Courbet,R. de Haas,N. Bethel,P. J. Leung,Timothy Huddy,S.J. Pellock,Doug Tischer,F. Chan,Brian Koepnick,H. Nguyen,Alex Kang,Banumathi Sankaran,Aloke Kumar Bera,Neil P. King,David Baker +21 more
TL;DR: The broad utility and high accuracy of ProteinMPNN is demonstrated using X-ray crystallography, cryoEM and functional studies by rescuing previously failed designs, made using Rosetta or AlphaFold, of protein monomers, cyclic homo-oligomers, tetrahedral nanoparticles, and target binding proteins.
Journal ArticleDOI
Scaffolding protein functional sites using deep learning
Jue Wang,Sidney Lisanza,David Juergens,Doug Tischer,Joseph L. Watson,Karla M Castro,Robert J. Ragotte,Amijai Saragovi,Lukas F. Milles,Minkyung Baek,Ivan Anishchenko,Wei Yang,Derrick R. Hicks,Marc Expòsit,Thomas Schlichthaerle,Jung Ho Chun,Justas Dauparas,N. Bennett,Basile I. M. Wicky,Andrew G. Muenks,Frank DiMaio,Bruno E. Correia,Sergey Ovchinnikov,David Baker +23 more
TL;DR: Wang et al. as mentioned in this paper proposed two deep learning methods to design proteins that contain prespecified functional sites, which can enable the scaffolding of desired functional residues within a well-folded designed protein.
Journal ArticleDOI
Protein sequence design by conformational landscape optimization
Christoffer Norn,Basile I. M. Wicky,David Juergens,Sirui Liu,David D. Kim,Doug Tischer,Brian Koepnick,Ivan Anishchenko,Foldit Players,David Baker,Sergey Ovchinnikov +10 more
TL;DR: In this article, the authors compare sequence design by conformational landscape optimization with the standard energy-based sequence design methodology in Rosetta and show that the former can result in energy landscapes with fewer alternative energy minima.
Journal ArticleDOI
Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling.
Doug Tischer,Orion D. Weiner +1 more
TL;DR: An optogenetic approach is developed to specifically tune the binding half-life of a chimeric antigen receptor without changing other binding parameters and provide direct evidence of kinetic proofreading in T cell signaling.