Photometric ninhydrin method for use in the chromatography of amino acids.

Publisher Summary This chapter discusses the chemical analysis of microbial cells. The preparation of material for analysis is discussed, because changes in the chemical composition of cells may occur as a result of the washing and storage conditions used. Wet- and dry-weight determinations of bacterial samples and assay of total cell numbers are described, because analytical results must refer to one or other of these values. Selection of an analytical procedure is a subjective process, because the number of suitable methods is large and each will have different merits and defects. Primary considerations are sensitivity, specificity, reproducibility, and absolute accuracy. Automatic methods for performing biochemical analyses, already widely accepted in hospitals and in industry, are beginning to make their way into the research laboratory. All automatic analyzers developed so far may be classified as either “continuous-flow” or “discrete” types. All of them use colorimetric methods exclusively and contain some form of automatic colorimeter for final read-out. The first and best-known is the Technicon “AutoAnalyzer,” which is of the continuous-flow type.

Chapter III Chemical Analysis of Microbial Cells

A Method for the Quantitative Modification and Estimation of Carboxylic Acid Groups in Proteins

Photometric estimation of proline and ornithine.

The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.

Gasometric determination of carboxyl groups in free amino acids

Estimation of formaldehyde in biological mixtures

Determination of free amino acids by titration of the carbon dioxide formed in the reaction with ninhydrin

The gasometric determination of amino acids in urine by the ninhydrin-carbon dioxide method

The determination of hydroxylysine in proteins

Douglas A. MacFadyen

Papers

Gasometric determination of carboxyl groups in free amino acids

Estimation of formaldehyde in biological mixtures

Determination of free amino acids by titration of the carbon dioxide formed in the reaction with ninhydrin

The gasometric determination of amino acids in urine by the ninhydrin-carbon dioxide method

The determination of hydroxylysine in proteins