Estimation of formaldehyde in biological mixtures
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This article is published in Journal of Biological Chemistry.The article was published on 1945-03-01 and is currently open access. It has received 358 citations till now.read more
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A Method for the Quantitative Modification and Estimation of Carboxylic Acid Groups in Proteins
D.G. Hoare,Daniel E. Koshland +1 more
TL;DR: The kinetics of the reaction indicates that there are limits to the variation of carbodiimide and nucleophile when quantitative modification is desired, but that a wide variety of reagents can be used if quantitative reaction is not essential.
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The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.
Journal ArticleDOI
Structure of the O-glycosidically linked carbohydrate units of fetuin.
Robert G. Spiro,V D Bhoyroo +1 more
TL;DR: Fetuin isolated from fetal calf serum was shown to contain 3 carbohydrate units which are attached to serine and threonine residues on the peptide chain and are distinct from the 3 asparagine-linked heteropolysaccharides previously reported to occur in this protein.
Journal ArticleDOI
Preparation of unilamellar liposomes of intermediate size (0.1–0.2 μm) by a combination of reverse phase evaporation and extrusion through polycarbonate membranes
Francis C. Szoka,Fred C. Olson,Timothy D. Heath,W.J. Vail,Eric Mayhew,Demetrios Papahadjopoulos +5 more
TL;DR: The procedure is relatively simple, rapid, and yields almost quantitative recovery of vesicles that encapsulate a large percentage of the total aqueous space.
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Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins.
Lee Van Lenten,Gilbert Ashwell +1 more
TL;DR: Evidence is presented to establish the identity of the radioactive derivative as 5-acetamido-3, 5-dideoxy-l-arabino-2-heptulosonic acid and to indicate it as the sole site of tritium incorporation in the carbohydrate chain.
References
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Gasometric determination of carboxyl groups in free amino acids
TL;DR: The method here presented depends on the fact that a-amino acids, when boiled in water with an excess of ninhydrin (triketohydrindene hydrate) at pH 1 to 5, evolve the CO2 of their carboxyl groups quantitatively in a few minutes.
Journal ArticleDOI
The gasometric determination of free amino acids in blood filtrates by the ninhydrin-carbon dioxide method
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The Determination of Hydrogen Ions
TL;DR: In this paper, a two-phase liquid-metal magnetohydrodynamic (MHD) generator is used to eliminate dissipative boundary layer electrical shunts by displacing the slow moving, conducting liquid boundary layer adjacent the insulating walls of the generator with a thin gas layer.
Journal ArticleDOI
Reaktionen und Reagenzien zum Nachweis organischer Verbindungen IV)
TL;DR: In this paper, a verwendete Apparat zeigt ihr gegeniiber gewisse Vereinfachungen ; die Fiillung mit Natronasbest and Phosphorpentoxyd ermSgliehte eine bedeutende Verkleinerung des Absorptionsrohres, wodureh eine besonders groge W~gegenauigkeit erzielt werden konnte; diese Verbesserungen, die fiber den Rahmen
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