Dwight R. Stoll

TL;DR: This report is deliberately focused on the issues involved in doing fast 2DLC by means of elevating the column temperature; however, many issues of broader applicability will be discussed.

TL;DR: There simply is not enough room in LC chromatograms to separate very many compounds that behave "statistically" and the attainable peak capacity does not suffice to separate complex samples, so LC cannot easily deal with complex mixtures that contain more than a few dozen analytes.

TL;DR: This tutorial discusses the motivations for doing two-dimensional liquid chromatography and describes the commonly used implementations of the method, and discusses the state of the art in 2D-LC performance as measured by peak capacity.

TL;DR: This work determination of the effective first-dimension peak width using the number of observed peaks in the entire 2D separation as the defining metric of performance is very useful in making realistic corrections to theoretical 2D peak capacities, and in guiding the optimization of 2D separations.

TL;DR: Compelling illustrations of the analytical potential of fast, high temperature 2DLC are evident in the clear presence of nine distinct peaks in a single second dimension chromatogram from a single quite narrow first dimension peak, and the great power of 2D LC to solve the "analytic dynamic range" problem inherent in the measurement of small peaks that are neighbors to a gigantic peak.