E. N. Lysogorskaya

TL;DR: This substrate was successfully applied to monitor thiol proteinase affinity chromatography on bacitracin-Sepharose, which resulted in a 2- to 4-fold purification from commercial preparations.

TL;DR: The serine proteinase secreted by the halophilic bacterium should be considered as a functional analogue, and a structural homologue, of eubacterial serineproteinases (subtilisins), indicating that the enzyme belongs to the subtilisin family.

TL;DR: Serine proteinase subtilisin 72 was covalently attached to the beads of poly(vinyl alcohol)-cryogel, a macroporous hydrogel prepared by the freeze-thaw technique and catalyzed with high yield the formation of peptide bonds between Phe-pNA and acyl donors.

TL;DR: Via a combination of chemical and enzymatic synthesis, new hexapeptide substrates convenient for use in activity assessment of several aspartyl proteinases--porcine pepin, human pepsin, gastricsin, and cathepsin D--were prepared.

TL;DR: It is proposed that the evolutionary conservation of cysteine peptidase in the complement of digestive peptidases of tenebrionid stored-product beetles is due not only to the adaptation of insects to plants rich in serine peptide inhibitors, but also to accommodate the need to efficiently cleave major dietary proteins rich in glutamine.