Showing papers in "Analytical Biochemistry in 1996"

TL;DR: The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.

TL;DR: A computer program with the code name DYNAFIT was developed for fitting either the initial velocities or the time course of enzyme reactions to an arbitrary molecular mechanism represented symbolically by a set of chemical equations.

TL;DR: An approach for real-time DNA sequencing without the need for electrophoresis has been developed that relies on the detection of DNA polymerase activity by an enzymatic luminometric inorganic pyrophosphate (PPi) detection assay (ELIDA) and the possibility for parallel processing of many samples in an automated manner is discussed.

TL;DR: Under standard assay conditions, the ratio of the absorbances, 590 nm over 450 nm, is strictly linear with protein concentration, and a linear equation that perfectly fits the experimental data is provided on the basis of mass action and Beer's law.

TL;DR: This review compares various methods of obtaining structural information from CD data and the advantages and pitfalls of each technique are detailed.

TL;DR: Overall, the technologies described here represent a significant advance toward faster, more automated, and more detailed glycan analysis.

TL;DR: Techniques of using size-exclusion chromatography with on-line light-scattering, uv absorbance, and refractive index detectors to characterize the polypeptide molecular weights of simple proteins or glycoproteins or to determine the stoichiometry of protein complexes are described.

TL;DR: The simultaneous determination of the biologically most interesting phytoestrogens and lignans in foods has not been carried out previously and the method will be useful for screening of important foods in populations with different risk of cancer and coronary heart disease, and for metabolic studies.

TL;DR: It is shown that binding constants in solution can be reproduced well by using on-rate determinations of antibody preincubated with antigen, and the conditions under which such an approach is valid are derived.

TL;DR: A new procedure that enables selective detection and sequencing of Ser-, Thr-, and Tyr-phosphopeptides at the low femtomole level in protein digests is described, and the stoichiometry of phosphorylation at specific sites is estimated from the ratio of the ion signals for the respective forms of the peptides observed in the normal full-scan mass spectra of the digest.

TL;DR: Two new fluorescent dyes are developed, SYPRO Orange protein gel stain and SYPRO Redprotein gel stain, to detect proteins in electrophoretic gels, allowing multiple photographs of gels to be taken without significant signal reduction.

TL;DR: This work proposes a strategy that introduces the equalization of concentrations (normalization) of specific transcripts during the subtractive process, which makes possible obtaining both rare and highly abundant transcripts in the resulting subtracted library.

TL;DR: Results suggest that the most common source of deviations from the pseudo-first-order kinetic approximation of BIAcore kinetic data is likely to be heterogeneity of the immobilized ligand sites.

TL;DR: The calibration constants for the oxygen-dependent quenching of the phosphorescence of Pd-meso-tetra (4-carboxyphenyl) porphine were determined as a function of albumin concentration, ionic strength in medium, pH, and temperature.

TL;DR: The attention is focused on the state-of-the-art, which is featured by a discriminant function algorithm developed very recently as well as an improved database established according to new experimental results.

TL;DR: Improved derivatization was obtained with the troublesome amino acids His and Tyr: exclusively monosubstituted His and disubst ituted Tyr were formed, eluting as free peaks in the chromatogram, thus permitting automated analysis of multiple samples.

TL;DR: A new kinetic analysis of biosensor data is presented, based upon a phenomenological two-compartment approximate description of transport, which permits determination of the correct values of chemical rate constants that are 10- to 100-fold greater than those that can be correctly evaluated by previous analyses.

TL;DR: The peroxynitrite in these postprocessed solutions has a broad absorption spectrum with a maximum around 302 nm, follows a characteristic first-order decomposition at pH 7.2 and 25°C, and reacts with organic compounds to give either nitrated or one-electron transfer products.

TL;DR: A dramatic improvement in the analytical sensitivity of this method is reported and it is found that the chemical specificity of the cyclocondensation reaction is not restricted to isothiocyanates, but includes dithiocarbamates, and related thiOCarbonyl compounds such as carbon disulfide, certain substituted thiourea derivatives, and xanthates.

TL;DR: High-resolution capillary gel electrophoresis was used for the separation of oligosaccharides labeled with a novel fluorophore 1-aminopyrene-3,6,8-trisulfonate at the reducing termini by reductive amination, achieving labeling efficiency greater than 97% with no significant loss of sialic acid residues.

TL;DR: It is demonstrated that complex membrane protein mixtures can be resolved with a resolution at least fivefold higher than that of one-dimensional SDS-PAGE.

TL;DR: Three-step chromatographic procedure facilitates the preparation of 120 mg or more of dityrosine from the enzyme-catalyzed oxidation of tyrosine, and two new reverse-phase HPLC techniques expedite the analysis of picomole quantities of dithrosine.

TL;DR: The accuracy of the secondary structure prediction from an infrared spectra data base of 39 proteins with known X-ray structure was investigated by different methods of multivariate data analysis and the hydrogen-bonded turns or bridges are predicted with higher accuracy than the assigned secondary structure types helix and beta-sheet.

TL;DR: Depsipeptide substrates incorporating an ester bond between residues P1 and P&prime1 are developed, enabling detection of activity with subnanomolar NS3 concentrations and the suitability of the assay for evaluation of inhibitors was established.

TL;DR: Synthetic glycopeptides were generated and used to demonstrate that O-GlcNAc-modified peptides can be rapidly identified in complex mixtures by HPLC-coupled electrospray mass spectrometry and to conclude that the selectivity and the sensitivity of this method will make it a powerful tool for determining the sites of O- Glc NAc modification on proteins of low abundance such as transcription factors and oncogenes.

TL;DR: The development of colorimetric and fluorometric methods for the reliable quantitation of S-nitrosothiols, utilizing conventional spectroscopic techniques available in most laboratories are reported.

TL;DR: Previously applied quantification methods for milk oligosaccharides based on gel permeation chromatography have been decisively improved.