Showing papers by "Edwin E. Budzinski published in 2001"
TL;DR: This review surveys the work that has been done on free radical-induced DNA double lesions and the potential biological significance of double lesions is discussed.
118 citations
TL;DR: The goal of the present investigation was to determine whether Fenton chemistry can generate the same kind of lesions as ionizing radiation, and the products were characterized by chromatography and by mass spectrometry.
Abstract: Patrzyc, H. B., Dawidzik, J. B., Budzinski, E. E., Iijima, H. and Box, H. C. Double Lesions are Produced in DNA Oligomer by Ionizing Radiation and by Metal-Catalyzed H2O2 Reactions. It was demonstrated previously that double lesions are produced in DNA by ionizing radiation. These double lesions consist of adjacent nucleotides each bearing a modified base. The goal of the present investigation was to determine whether Fenton chemistry can generate the same kind of lesions. DNA oligomers were exposed to metal-catalyzed H2O2 reactions, and the products were characterized by chromatography and by mass spectrometry. Double lesions are produced by this treatment in which deoxyguanosine is oxidized to 8-oxo-7,8- dihydrodeoxyguanosine and an adjacent pyrimidine nucleoside is degraded to a formamido remnant.
19 citations
TL;DR: The present study shows that double lesions may sometimes consist of a base lesion and an associated strand break and the mechanism for the formation of double lesions is discussed.
Abstract: Box, H. C., Budzinski, E. E., Dawidzik, J., Patrzyc, H. B. and Freund, H. G. A Novel Double Lesion in X-Irradiated DNA Consists of a Strand Break and a Base Modification. Radiat. Res. 156, 215–219 (2001). A single free radical-initiating event can produce a pair of base lesions in DNA oligomers exposed to ionizing radiation. Whereas double base lesions have been observed previously, the present study shows that double lesions may sometimes consist of a base lesion and an associated strand break. The mechanism for the formation of double lesions is discussed. A redox process is postulated in which guanine is the source of the electron. It is suggested that double lesions may be formed in DNA either on adjacent nucleotides or, alternatively, on nucleotides separated by one, two or possibly more intervening nucleotides. It is hypothesized that intramolecular electron transfer facilitates the formation of double lesions on nonadjacent nucleotides.
13 citations
01 Jan 2001
TL;DR: Box et al. as mentioned in this paper showed that double lesions may sometimes consist of a base lesion and an associated strand break, and a redox process is postulated in which guanine is the source of the electron.
Abstract: Box, H. C., Budzinski, E. E., Dawidzik, J., Patrzyc, H. B. and Freund, H. G. A Novel Double Lesion in X-Irradiated DNA Consists of a Strand Break and a Base Modification. Radiat. Res. 156, 215‐219 (2001). A single free radical-initiating event can produce a pair of base lesions in DNA oligomers exposed to ionizing radiation. Whereas double base lesions have been observed previously, the present study shows that double lesions may sometimes consist of a base lesion and an associated strand break. The mechanism for the formation of double lesions is discussed. A redox process is postulated in which guanine is the source of the electron. It is suggested that double lesions may be formed in DNA either on adjacent nucleotides or, alternatively, on nucleotides separated by one, two or possibly more intervening nucleotides. It is hypothesized that intramolecular electron transfer facilitates the formation of double lesions on nonadjacent nucleotides. q 2001 by Radiation Research Society
3 citations