Eileen Bauer

TL;DR: Repeated, noninvasive, and quantitative imaging of PET reporter gene expression should be a valuable tool for studies of human gene therapy, of organ/cell transplantation, and of both environmental and behavioral modulation of gene expression in transgenic mice.

TL;DR: The use of HSV1-sr39tk as a PET reporter gene and FPCV as aPET reporter probe results in significantly enhanced sensitivity for imaging reporter gene expression in vivo.

TL;DR: The feasibility of using [8-14C]-ganciclovir as a reporter probe for the HSV1-tk reporter gene, using an in vivo adenoviral mediated gene delivery system in a murine model is demonstrated.

TL;DR: Results indicate that FPCV is a better reporter probe than is FGCV for imaging lower levels of HSV1 -tk gene expression in vivo and reveal the ability to monitor the expression of two distinct reporter genes in the same animal using reporter probes specific for each gene.

TL;DR: Reporter genes are emerging as very powerful tools to monitor the delivery, magnitude, and time variation of therapeutic gene transfer in vivo and applications of the reporter gene technology to other areas, such as cell trafficking studies and transgenic animal models are now possible.