Molecularly imprinted polymers and their use in biomimetic sensors.

Advanced functional polymer membranes

https://www.uta.edu/rfmems/GI_Sensor/papers/ac040049d.pdf

Fiber-Optic Chemical Sensors and Biosensors

Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003

Conducting polymers in chemical sensors and arrays

New types of polymeric membranes with molecular recognition sites for l-phenylalanine (l-Phe), 6-amino-1-propyluracil (APU), atrazine, and sialic acid have been prepared using the molecular imprinting approach. The membrane synthesis includes radical polymerization of ethylene glycol dimethacrylate (EDMA) and functional monomers in the presence of a template. Several compounds(diethylamino)ethyl methacrylate (DEAEM), methacrylic acid (MAA), allylamine (AA), and (4-vinylphenyl)boronic acidwere as functional monomers, which are able to form covalent, ionic, or hydrogen bonds with the corresponding templates. Template specific conductometric sensors, based on these polymers, were constructed and studied. An opposite response of covalently versus noncovalently imprinted membranes was demonstrated and discussed in detail. Sensors based on these materials could detect the target molecules at concentrations of 1−50 μM in solution. The high specificity and stability of these imprinted membranes render them promis...

Imprinted membranes for sensor technology : Opposite behavior of covalently and noncovalently imprinted membranes

Thylakoid membranes-based test-system for detecting of trace quantities of the photosynthesis-inhibiting herbicides in drinking water

The optoelectronic biosensor, based on surface plasmon resonance (SPR) for detection of photosynthesis-inhibiting pesticides in aqueous solutions was presented. The pesticide capability to replace plastoquinone from its complex with D1 protein was used for the detection. This replacement reaction results in the changes of the optical characteristics of protein layer, immobilized on the gold surface. Monitoring of these changes with SPR-technique permits to determine 0.05–5.0 μg/ml pesticide in solution within 1 h.

Surface plasmon resonance sensor for pesticide detection

A technique for coating the wells of microtiterplates with polyaniline layers and with polyaniline/enzyme layers is presented. The resulting wells are shown to be useful for assaying enzyme substrates (as exemplified for glucose via pH) and hydrogen peroxide (via the redox properties of the film). Analyte detection is based on monitoring the absorption spectra of the polyaniline, which turn purple as a result of redox processes, or green on formation of acids by enzymatic reactions. Hydrogen peroxide (a species produced by all oxidases) and glucose (which yields protons on enzymatic oxidation) have been determined in the millimolar to micromolar concentration range. High sensitivity, film stability and good reproducibility of the measurements make the system an attractive alternative to existing biosensing schemes.

Elena V. Piletskaya

Papers

Imprinted membranes for sensor technology : Opposite behavior of covalently and noncovalently imprinted membranes

Thylakoid membranes-based test-system for detecting of trace quantities of the photosynthesis-inhibiting herbicides in drinking water

Surface plasmon resonance sensor for pesticide detection

Polyaniline-coated microtiter plates for use in longwave optical bioassays.

D1 protein – an effective substitute for immunoglobulins in ELISA for the detection of photosynthesis inhibiting herbicides