Showing papers by "Elisabetta Meucci published in 1989"
TL;DR: In this paper, it was shown that the strong efficiency of ascorbate quenching is inconsistent with a collisional type of mechanism and that the mechanism of the process is static (formation of a complex) and not dynamic.
1 citations
01 Jan 1989
TL;DR: Cobalt can replace zinc yielding a partially active phosphohydrolase, which lacks phosphotransferase activity and presents a relevant visible absorption in bovine kidney alkaline phosphatase.
Abstract: Alkaline phosphatase (EC 3.1.3.1), a zinc metallo-enzyme, is irreversibly inhibited by ascorbate, probably through a modification of active site environment (Martorana et al., 1986; Miggiano et al., 1984). A metal substituted form was prepared from bovine kidney alkaline phosphatase purchased from Calbiochem (La Jolla, CA, U.S.A.) and further purified as described (Martorana et al., 1986). Cobalt can replace zinc yielding a partially active phosphohydrolase, which lacks phosphotransferase activity (Ensinger et al., 1978) and presents a relevant visible absorption (Simpson and Vallee, 1968).