E
Elizabeth Kimball
Researcher at Princeton University
Publications - 8
Citations - 3482
Elizabeth Kimball is an academic researcher from Princeton University. The author has contributed to research in topics: Metabolite & Metabolome. The author has an hindex of 8, co-authored 8 publications receiving 3159 citations.
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Journal ArticleDOI
Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli
Bryson D. Bennett,Elizabeth Kimball,Melissa Gao,Robin E. Osterhout,Stephen J. Van Dien,Joshua D. Rabinowitz +5 more
TL;DR: The data and analyses presented here highlight the ability to identify organizing metabolic principles from systems-level absolute metabolite concentration data, and facilitate efficient flux reversibility given thermodynamic and osmotic constraints.
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Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry.
TL;DR: A liquid chromatography-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for reliable measurement of 141 metabolites, including components of central carbon, amino acid, and nucleotide metabolism is presented.
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Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach
TL;DR: This protocol provides a method for quantitating the intracellular concentrations of endogenous metabolites in cultured cells using Escherichia coli and primary human fibroblasts fed uniformly with 13C-labeled carbon sources and enables absolute quantitation of several dozen metabolites over ∼1 week of work.
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Acidic Acetonitrile for Cellular Metabolome Extraction from Escherichia coli
TL;DR: Experiments involving isotopic tracers revealed that the improved triph phosphate yields in the acidic acetonitrile were in part due to reduced triphosphate decomposition, which is a major problem when extracting with other solvent systems such as methanol/water.
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Conservation of the metabolomic response to starvation across two divergent microbes
Matthew J. Brauer,Jie Yuan,Bryson D. Bennett,Wenyun Lu,Elizabeth Kimball,David Botstein,Joshua D. Rabinowitz +6 more
TL;DR: Following 68 cellular metabolites after carbon or nitrogen starvation of Escherichia coli and Saccharomyces cerevisiae, using a filter-culture methodology that allows exponential growth, nondisruptive nutrient removal, and fast quenching of metabolism, captured a remarkable 72% of the metabolite concentration changes.