Stephen J. Van Dien

TL;DR: The data and analyses presented here highlight the ability to identify organizing metabolic principles from systems-level absolute metabolite concentration data, and facilitate efficient flux reversibility given thermodynamic and osmotic constraints.

TL;DR: This work engineered the E. coli host to enhance anaerobic operation of the oxidative tricarboxylic acid cycle, thereby generating reducing power to drive the BDO pathway, leading to a strain of Escherichia coli capable of producing 18 g l(-1) of this highly reduced, non-natural chemical.

TL;DR: A sustainable bioprocess for the production of 1,4-butanediol from carbohydrate feedstocks was developed and an overall process that successfully performed at commercial scale for direct production of bio-BDO from dextrose is highlighted.

TL;DR: In this paper, the authors present the challenges in reaching commercial titer, yield, and productivity targets, along with other necessary strain and process characteristics, and present a strategy for using these tools to overcome the major hurdles on the path to commercialization.

TL;DR: A computational framework that combines a constraint-based modeling framework with isotopic label tracing on a large scale is presented that investigates the importance of carrying out isotopic labeling studies using a more comprehensive reaction network including global metabolite balances on cofactors such as ATP, NADH, and NADPH.