E
Eloi Garí
Researcher at University of Lleida
Publications - 52
Citations - 2975
Eloi Garí is an academic researcher from University of Lleida. The author has contributed to research in topics: Cell cycle & Cyclin. The author has an hindex of 22, co-authored 48 publications receiving 2642 citations. Previous affiliations of Eloi Garí include Autonomous University of Barcelona & Centre national de la recherche scientifique.
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A Set of Vectors with a Tetracycline-Regulatable Promoter System for Modulated Gene Expression in Saccharomyces cerevisiae
TL;DR: In this article, a set of Saccharomyces cerevisiae expression vectors has been developed in which transcription is driven by a hybrid tetO-CYC1 promoter through the action of a tetR-VP16 (tTA) activator.
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An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast.
TL;DR: An activator/repressor expression system for budding yeast in which tetracyclines control in opposite ways the ability of tetR-based activator and repressor molecules to bind tetO promoters may be useful for the functional analysis of essential genes whose conditional expression can be tightly controlled by tetrACYclines.
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The critical size is set at a single-cell level by growth rate to attain homeostasis and adaptation
Francisco Ferrezuelo,Neus Colomina,Alida Palmisano,Alida Palmisano,Eloi Garí,Carme Gallego,Attila Csikász-Nagy,Martí Aldea +7 more
TL;DR: It is found that a growth-rate-dependent sizer is sufficient to ensure size homeostasis and, as a remarkable advantage over a rigid sizer mechanism, it provides an immediate solution for size adaptation to external conditions at a population level.
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The Cln3 cyclin is down-regulated by translational repression and degradation during the G1 arrest caused by nitrogen deprivation in budding yeast
TL;DR: It is shown that the Cln3 cyclin, which has a key role in the timely activation of SBF (Swi4–Swi6)‐ and MBF‐dependent promoters in late G1, is down‐regulated rapidly at a post‐transcriptional level in cells deprived of the nitrogen source.
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Functional analysis of yeast essential genes using a promoter-substitution cassette and the tetracycline-regulatable dual expression system.
TL;DR: A promoter‐substitution cassette has been constructed that allows one‐step substitution of chromosomal gene promoters for the tetracycline‐regulatable tetO promoter in yeast cells, which uses kanMX4 as selective marker for geneticin resistance.